Environmental biosafety assessment on transgenic Oncidium orchid modified by RNA interference of Phytoene Synthase genes.

IF 1.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Pub Date : 2019-09-25 DOI:10.5511/plantbiotechnology.19.0814a

Swee-Suak Ko, Yao-Chung Liu, M. Chung, M. Shih, Habibi Mohammadmehdi, Taichi Oguchi, Kazuo N. Watanabe, K. Yeh

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引用次数: 3

Abstract

Hybrid Oncidium orchids, such as Oncidium Gower Ramsey and Oncidium "Honey Angel," are popular cut flowers in Japan and Taiwan. Due to pollen sterility, no new varieties have been created by conventional breeding methods. Recently, we employed RNA interference (RNAi) technology to suppress phytoene synthase and successfully modified floret hue from yellow to white (Liu et al. 2019). Transgenic white Oncidium orchids, Honey Snow MF-1, have been grown to test their genetic stability, and their environmental biosafety was assessed for approximately one year under government regulatory instructions from the Council of Agriculture, Taiwan. In the present study, pollen sterility was demonstrated by cytological observation of the microsporogenesis step, pollen morphology abortion, and failure of pollen germination. Assays on allelopathic effect on the other plants and the soil rhizospheric microbial flora-revealed that transgenic Oncidium orchids are potentially safe with regard to environmental biodiversity. Therefore, the general release permissions have been granted and an application for licensing for commercial production is under way.

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植物烯合酶基因RNA干扰转基因兰菖蒲的环境生物安全性评价。

在日本和台湾，杂交文心兰，如文心兰和“蜂蜜天使”文心兰是很受欢迎的鲜花。由于花粉不育，传统的育种方法还没有创造出新的品种。最近，我们采用RNA干扰（RNAi）技术抑制八氢番茄红素合成酶，并成功地将小花色调从黄色改为白色（Liu et al.2019）。转基因白文心兰（Honey Snow MF-1）已被种植以测试其遗传稳定性，并根据台湾农业委员会的政府监管指示，对其环境生物安全性进行了约一年的评估。在本研究中，通过对小孢子发生步骤、花粉形态败育和花粉发芽失败的细胞学观察，证明了花粉的不育性。对其他植物和土壤根际微生物区系的化感作用分析表明，转基因文心兰在环境生物多样性方面具有潜在的安全性。因此，已经授予了一般发布许可，商业生产的许可申请正在进行中。

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来源期刊

Plant Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-PLANT SCIENCES

CiteScore

2.90

自引率

18.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.