Physical and chemical fermentation conditions affect the growth and metabolite production of endophytic fungi Athelia rolfsii

IF 0.7 Q4 PHARMACOLOGY & PHARMACY Journal of Reports in Pharmaceutical Sciences Pub Date : 2022-01-01 DOI:10.4103/jrptps.JRPTPS_136_20
Ririn Suharsanti, S. Wahyuono, Puji Astuti
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Abstract

Background: The effort to explore antimicrobial agents isolated from an endophytic fungus of Coleus amboinicus resulted in the finding of a potential aromatic compound having methoxy, hydroxyl, and methyl groups produced by Athelia rolfsii. This compound exhibited antibacterial activities with IC50 values of <2 μg/mL against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Streptococcus mutans, and Pseudomonas aeruginosa. This study was aimed to determine the effect of varying fermentation conditions (types of media, carbon sources, nitrogen sources, temperature, and pH) on biomass, total metabolites, and bioactive compound production. Materials and Methods: The fungal cultures were subjected to various treatment conditions and incubated for 12 days at 25°C 160 rpm followed by analyzing the biomass, metabolite, and bioactive compound production. Results and Conclusion: The study found that although the maximum total metabolite production was achieved in the Tryptic Soy Broth medium, both biomass and bioactive compound production accumulated at the highest amount in Potato Dextrose Broth and Sabouraud Dextrose Broth media. Adding 1% of different types of carbon sources did not significantly enhance biomass, total metabolite, and bioactive compound production. Three types of organic nitrogen sources used in this study did not significantly affect biomass and total metabolite production, but adding peptone produced the highest amount of bioactive compound. Supplementing inorganic source of nitrogen to the culture medium decreased the production. While pH 5.5 was found to be the optimum condition for total metabolite production, pH 6–7 resulted in higher productivity of the bioactive compound. The total metabolite production was best produced at 25°C, whereas higher temperatures were needed to get optimum bioactive and biomass production. This study found that the total metabolite production was 7.8 times higher when the culture was grown in PDB medium supplemented with 1% sucrose and 1% peptone and incubated at 27°C at pH 6.5; whereas a 15% increase was observed in the bioactive compound production.
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物理和化学发酵条件对内生真菌罗氏Athelia rolfsii生长和代谢产物产生的影响
背景:为了探索从一种内生真菌Coleus amboinicus中分离出的抗菌剂,发现了一种潜在的具有甲氧基、羟基和甲基的芳香化合物。该化合物对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌、变形链球菌和铜绿假单胞菌具有IC50值<2μg/mL的抗菌活性。本研究旨在确定不同发酵条件(培养基类型、碳源、氮源、温度和pH)对生物量、总代谢产物和生物活性化合物产生的影响。材料和方法:将真菌培养物置于各种处理条件下,在25°C 160 rpm下孵育12天,然后分析生物量、代谢产物和生物活性化合物的产生。结果与结论:研究发现,尽管在胰蛋白酶大豆肉汤培养基中实现了最大的总代谢产物产量,但在马铃薯葡萄糖肉汤和沙氏葡萄糖肉汤中,生物量和生物活性化合物的产量积累最高。添加1%的不同类型的碳源并不能显著提高生物量、总代谢产物和生物活性化合物的产生。本研究中使用的三种有机氮源对生物量和总代谢产物的产生没有显著影响,但添加蛋白胨可产生最高量的生物活性化合物。在培养基中添加无机氮源降低了产量。虽然发现pH 5.5是产生总代谢产物的最佳条件,但pH 6-7可提高生物活性化合物的生产率。总代谢产物的产生最好在25°C时产生,而需要更高的温度才能获得最佳的生物活性和生物量产生。该研究发现,当培养物在添加1%蔗糖和1%蛋白胨的PDB培养基中生长并在27°C、pH 6.5下孵育时,总代谢产物产量高出7.8倍;而在生物活性化合物的生产中观察到15%的增加。
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来源期刊
Journal of Reports in Pharmaceutical Sciences
Journal of Reports in Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.40
自引率
0.00%
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0
期刊介绍: The Journal of Reports in Pharmaceutical Sciences(JRPS) is a biannually peer-reviewed multi-disciplinary pharmaceutical publication to serve as a means for scientific information exchange in the international pharmaceutical forum. It accepts novel findings that contribute to advancement of scientific knowledge in pharmaceutical fields that not published or under consideration for publication anywhere else for publication in JRPS as original research article. all aspects of pharmaceutical sciences consist of medicinal chemistry, molecular modeling, drug design, pharmaceutics, biopharmacy, pharmaceutical nanotechnology, pharmacognosy, natural products, pharmaceutical biotechnology, pharmacology, toxicology and clinical pharmacy.
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