Antibiotic resistance, virulence factors, and phylogenetic groups of Escherichia coli isolated from hospital wastewater: A case study in the west of Iran

IF 1.3 Q4 ENVIRONMENTAL SCIENCES Environmental Health Engineering and Management Journal Pub Date : 2023-04-01 DOI:10.34172/ehem.2023.15
A. Bozorgomid, Roya Chegene Lorestani, M. Rostamian, Fatemeh Nemati Zargaran, Zhila Shahvaisi-Zadeh, A. Akya
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Abstract

Background: The appropriate management of hospital wastewater is an essential process to prevent the spread of pathogenic strains of bacteria including Escherichia coli in this study, the antibiotic resistance, virulence characteristics, and phylogenetic diversity of E. coli isolated from the raw and treated hospital wastewater in a general hospital in the west of Iran were evaluated. Methods: E. coli isolates were recovered and identified using culture and biochemical tests. Sixty isolates were used and antimicrobial resistance, virulence genes, antibiotic-resistant genes (ARGs), and phylogeny groups of isolates were determined using polymerase chain reaction (PCR) assay. The antibiotic resistance was tested using disk diffusion. Results: The antibiotic susceptibility testing indicated that the resistance to co-trimoxazole was the most common, followed by ceftriaxone, amikacin, and gentamicin. Multi-drug resistance (MDR) was observed in 90% of raw and 96.66% of treated sewage isolates. The phylogeny groups B1 and A were the most common groups among isolates of raw and treated sewage, respectively. The most common virulence genes detected were sfa, papC, and fyuA; while pic and sepA genes were not found in the isolates. The most common ARGs were blaTEM (in 90% isolates of raw and 92.5% of treated sewage) and blaCTX-M (in 60% isolates of raw and 77.5% of treated sewage). The blaSHV-5 gene was not detected among isolates. Conclusion: The results highlight the potential of hospital wastewater as a source for spreading the virulent and multi drug-resistant strains of E. coli.
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从医院废水中分离的大肠杆菌的抗生素耐药性、毒力因子和系统发育群:伊朗西部的一个案例研究
背景:对医院废水进行适当的管理是防止包括大肠杆菌在内的病原菌传播的必要过程。本研究对伊朗西部一家综合医院未经处理的医院废水中分离的大肠杆菌的抗生素耐药性、毒力特征和系统发育多样性进行了评估。方法:采用培养和生化试验对分离的大肠杆菌进行回收和鉴定。采用聚合酶链反应(PCR)法对60株菌株进行耐药性、毒力基因、耐药基因(ARGs)和系统发育类群测定。采用纸片扩散法检测抗生素耐药性。结果:药敏试验结果显示,耐药最多的是复方新诺明,其次是头孢曲松、阿米卡星和庆大霉素。90%的生分离菌株和96.66%的处理过的污水分离菌株存在多药耐药(MDR)。系统发育类群B1和A分别是原污水和处理污水分离株中最常见的类群。最常见的毒力基因为sfa、papC和fyuA;而在分离株中未发现pic和sepA基因。最常见的ARGs是blaTEM(90%的分离株和92.5%的处理污水)和blaCTX-M(60%的分离株和77.5%的处理污水)。分离株中未检出blaSHV-5基因。结论:医院废水有可能成为大肠杆菌强毒和多重耐药菌株的传播源。
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来源期刊
CiteScore
2.40
自引率
37.50%
发文量
17
审稿时长
12 weeks
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