Determination of Antioxidant Activity and Phenolic Compounds of Methanolic Extract of Java Plum (Syzygium cumini Linn. (Skeel) Seed

Indonesian Food and Nutrition Progress Pub Date : 2017-08-31 DOI:10.22146/IFNP.24279

R. Rohadi, U. Santoso, S. Raharjo, I. Falah

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引用次数: 8

Abstract

Methanolic extract of Java Plum ( Syzygium cumini L. (Skeel) seed (MEJS) is potential source of natural antioxidant. As indicated by several in vitro measurements, the extract had strong DPPH (1,1 diphenyl, 2–picryl hydrazyl) and ABTS (2,2-azinobis, 3-ethylbenzothiazoline-6-sulphonate) radical scavenging activity, strong Ferric Reducing Antioxidant Power (FRAP) and moderate inhibition activity of linoleic acid oxidation. This study aimed to determine antioxidant activity and phenolic compound of Java Plum seed ( Syzygium cumini L. (Skeel) methanolic extract fractions. Phenolics compound identification using Thin Layer Chromatography (TLC) showed that all fractions (polar, semi polar and hydrolyzed semi polar fraction) contained Gallic acid, Tannic acid and flavonol’s Rutin. HPLC-DAD analysis showed that its polar fraction contained 25 ppm flavonol’s Quercetine and 55181 ppm flavonol’s (+)- Catechin, ethyl acetate fraction contained 54 ppm flavonol’s Rutin and 528 ppm (+)- Catechine, while hydrolyzed ethyl acetate fraction contained 404 ppm Rutin and 28692 ppm (+)- Catechine.

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爪哇梅甲醇提取物抗氧化活性及酚类化合物的测定。(斯基尔)种子

爪哇梅（Syzygium cumini L.（Skeel）籽（MEJS）的甲醇提取物是天然抗氧化剂的潜在来源。几项体外测定表明，该提取物具有较强的DPPH（1，1-二苯，2-苦基肼基）和ABTS（2，2-锌二苯，3-乙基苯并噻唑啉-6-磺酸酯）自由基清除活性、较强的铁还原抗氧化能力（FRAP）和中等的亚油酸氧化抑制活性。本研究旨在测定Java Plum seed（Syzygium cumini L.（Skeel）甲醇提取物的抗氧化活性和酚类化合物。薄层色谱法（TLC）对酚类化合物的鉴定表明，所有组分（极性、半极性和水解半极性组分）均含有没食子酸、单宁酸和黄酮醇芦丁。HPLC-DAD分析表明，其极性部分含有25ppm黄酮醇槲皮素和55181ppm黄酮醇（+）-儿茶素，乙酸乙酯部分含有54ppm黄酮醇芦丁和528ppm（+）-Catechine，而水解乙酸乙酯部分包含404ppm芦丁和28692ppm（+。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Indonesian Food and Nutrition Progress

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8 weeks