{"title":"The effect of sex hormone-binding globulin gene polymorphisms on the serum level of SHBG hormone in the men with prostate cancer","authors":"Zahra Tahmasebi Fard","doi":"10.1016/j.mgene.2021.101000","DOIUrl":null,"url":null,"abstract":"<div><p><span>To evaluated the possible correlations of prostate cancer and three coding region polymorphisms (rs 6257, rs6258, rs 6259), & changing repeat of TAAAA in promoter of SHBG gene regarding to concentration of Prostate specific Antigen and SHBG levels. For study purpose, a total of 352 subjects (176 patients and 176 controls) were recruited. The </span>ELISA<span> technique and the RFLP-PCR method were used. Logistic and adjusted regression of genotypes showed that the (TAAAA)n polymorphism in individuals carrying more than five repeats of the five-nucleotide sequence of TAAAA, as well as AA+AG (rs6257) and AA+AG (rs6259), increased the risk of prostate cancer by the chance ratio of 1.622, 2.005, and 2.469, respectively. The studied polymorphisms showed a significant relationship with the stage of the disease and Gleason score. The individuals carrying the mutants were less likely to develop prostate cancer than the ones with wild genotypes. All the genotypes in the cancer group had higher serum levels of tPSA and fPSA than those in the control group. A significant association was observed (except tPSA and fPSA for GT (rs6258) and fPSA for GA (rs6259)). Serum level of SHBG also showed a significant correlation with prostate cancer in genotypes >Panta TAAAA (TAAAA)n, GG (rs6257), TT and TT + CT (rs6258), and GG, AA, and AA+GA (rs6259). These polymorphisms by changing the serum levels SHBG contributed to the incidence of prostate cancer.</span></p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"31 ","pages":"Article 101000"},"PeriodicalIF":0.8000,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Meta Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214540021001511","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
To evaluated the possible correlations of prostate cancer and three coding region polymorphisms (rs 6257, rs6258, rs 6259), & changing repeat of TAAAA in promoter of SHBG gene regarding to concentration of Prostate specific Antigen and SHBG levels. For study purpose, a total of 352 subjects (176 patients and 176 controls) were recruited. The ELISA technique and the RFLP-PCR method were used. Logistic and adjusted regression of genotypes showed that the (TAAAA)n polymorphism in individuals carrying more than five repeats of the five-nucleotide sequence of TAAAA, as well as AA+AG (rs6257) and AA+AG (rs6259), increased the risk of prostate cancer by the chance ratio of 1.622, 2.005, and 2.469, respectively. The studied polymorphisms showed a significant relationship with the stage of the disease and Gleason score. The individuals carrying the mutants were less likely to develop prostate cancer than the ones with wild genotypes. All the genotypes in the cancer group had higher serum levels of tPSA and fPSA than those in the control group. A significant association was observed (except tPSA and fPSA for GT (rs6258) and fPSA for GA (rs6259)). Serum level of SHBG also showed a significant correlation with prostate cancer in genotypes >Panta TAAAA (TAAAA)n, GG (rs6257), TT and TT + CT (rs6258), and GG, AA, and AA+GA (rs6259). These polymorphisms by changing the serum levels SHBG contributed to the incidence of prostate cancer.
Meta GeneBiochemistry, Genetics and Molecular Biology-Genetics
CiteScore
1.10
自引率
0.00%
发文量
20
期刊介绍:
Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.