Alpha-amylase Inhibitory Activity of Extracts from Algerian Calamintha nepeta (L.)

Q4 Pharmacology, Toxicology and Pharmaceutics Current Enzyme Inhibition Pub Date : 2023-03-31 DOI:10.2174/1573408019666230331084001

K. Benabed, Hadjer Boussoussa, Ihcen Khacheba, Abir Bekhaoua, Fatima zohra Douadji, sabrina Daïdi, Souad Djaafour, M. Yousfi

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Abstract

Calamintha nepeta is a plant from the Lamiaceae family that is known for its traditional use to treat diabetes among Algerian populations. However, till now, there has been no research work to confirm this activity. Calamintha nepeta is a plant from the Lamiaceae family which is known for its traditional use to treat diabetes in Algerian populations. Until now there have been no research work to confirm this activity. This work aimed to evaluate the amylase inhibitory activity of essential oils and phenolic compounds from both methanolic and aqueous extracts of Algerian Calamintha nepeta (L.). The evalutation of the antiamylatic activity of essential oil, methanolic and aqueous extracts from Algerian Calamintha nepeta (L.). The essential oil was obtained by hydrodistillation and analyzed using GC and then GC/MS. Aqueous and methanolic extracts were obtained from the remains of the hydrodistillation. Total phenolic compounds were quantified using the Folin-Ciocalteu method. The amylase inhibitory activity of the extracts was determined by testing their ability to inhibit alpha-amylase. The extraction yield was 0.67 % (w/w) for the essential oil, and 11.85 and 4.38 % (w/w) for the aqueous and methanolic extracts, respectively. The essential oil analysis revealed that menthone, menthol, pulegone, and pulegone oxide were the main components of the oil. The total phenolic compounds in the aqueous and methanolic extracts were 41.81 and 32.92 mg GAE/g DW, respectively. The extracts inhibited α-amylase activity with IC50 values of 24.46, 31.54, and 115.47 mg/ml for the methanolic extract, essential oil, and aqueous extract, respectively. Extraction yield was 0.67 %(w/w) for the essential oil, and 11.85 and 4.38 %(w/w) for the aqueous and methanolic extracts respectively. The essential oil analysis revealed that menthone, menthol, pulegone and pulegone oxide were the main components. Total phenolic compounds for the aqueous and methanolic extracts were respectively 41.81 and 32.92 mg GAE/g. The different extracts showed inhibition activity towards α-amylase with IC50 values equal to : 24.46 ; 31.54 and 115.47mg/ml for the methanolic extract, the essential oil and the aqueous extract respectively. The different extracts of Calamintha nepeta showed an interesting composition and significant amylase inhibitory activity, emphasizing their successful use in traditional medicine. The different extracts of Calamintha nepeta showed an interesting composition, and a significant antimaylatic activity. future profound studies are necessary to identify the active molecules

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阿尔及利亚菖蒲提取物抑制α -淀粉酶活性的研究

Calamintha nepeta是Lamiaceae科的一种植物，以其在阿尔及利亚人群中治疗糖尿病的传统用途而闻名。然而，到目前为止，还没有研究工作证实这一活动。Calamintha nepeta是Lamiaceae科的一种植物，以其在阿尔及利亚人群中治疗糖尿病的传统用途而闻名。到目前为止，还没有任何研究工作来证实这种活动。本工作旨在评价阿尔及利亚水菜（Calamintha nepeta（L.））甲醇提取物和水提取物中精油和酚类化合物的淀粉酶抑制活性。)。通过加氢蒸馏获得精油，并使用GC和GC/MS进行分析。从加氢蒸馏的残余物中获得水提取物和甲醇提取物。使用Folin-Ciocalteu方法对总酚类化合物进行定量。通过测试提取物抑制α-淀粉酶的能力来测定提取物的淀粉酶抑制活性。精油的提取率为0.67%（w/w），水提取物和甲醇提取物的提取率分别为11.85%和4.38%（w/w）。精油分析表明，薄荷酮、薄荷醇、普勒酮和氧化普勒酮是精油的主要成分。水提取物和甲醇提取物中的总酚类化合物分别为41.81和32.92 mg GAE/g DW。提取物抑制α-淀粉酶活性，甲醇提取物、精油和水提取物的IC50值分别为24.46、31.54和115.47mg/ml。精油的提取率为0.67%（w/w），水提取物和甲醇提取物的提取率分别为11.85%和4.38%（w/w）。精油分析表明，薄荷酮、薄荷醇、普勒酮和氧化普勒酮是主要成分。水提取物和甲醇提取物的总酚类化合物分别为41.81和32.92 mg GAE/g。不同提取物对α-淀粉酶均有抑制作用，IC50值为：24.46；甲醇提取物、精油和水提取物分别为31.54和115.47mg/ml。荆芥的不同提取物表现出有趣的组成和显著的淀粉酶抑制活性，强调了它们在传统医学中的成功应用。荆芥的不同提取物显示出有趣的组成和显著的抗疟活性。需要进一步深入的研究来鉴定活性分子

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来源期刊

Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.