P. Promtan, J. Panatuk, W. Kongbuntad, D. Amornlerdpison, Y. Nanta, N. Pripwai, W. Thaworn, W. Pattanawong
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引用次数: 0
Abstract
This research was conducted to investigate the growth performance of black-boned chicken embryonic stem (ES) cells for the future development of cultured meat. Black-boned chicken ES cells were isolated and cultured from fertilized eggs. The treatments applied were: fetal bovine serum (FBS) (T1), commercial chicken serum (SCK) (T2), Pradu Hang Dam chicken serum (PDC) (T3), and black-boned chicken serum (BBC) (T4). Black-boned chicken ES cells were cultured at 37.0 °C in a humidified environment of 5% CO 2 , for 10 days. The growth of black-boned chicken ES cells concentration was measured by the absorbance at 450 nm. A haemocytometer was used to count the number of black-boned chicken ES cells. Comparing the protein content of cultured meat and chicken meat was collected for combustion and proximate analysis. All collected data were analyzed using ANOVA in a completely randomized design. T4 tended to have a higher number and growth rate than the other groups, followed by T3, T2, and T1, respectively. When counted ES cells final, T4 had a significantly higher number and growth rate than the other groups (p<0.001). Comparing the protein content, it was found that cultured meat had significantly more protein than the other groups (p<0.001), and characteristics (alignment and cross-section of muscle fibers) were not different from chicken cells. Finally, the cultivation of black-boned chicken ES cells necessitates the use of a medium containing black-boned chicken serum in the growth and development of black-boned Chicken ES cells for culture meat.
本研究旨在研究乌骨鸡胚胎干细胞(ES)的生长性能,为培养肉的未来发展提供参考。从受精卵中分离和培养黑骨鸡ES细胞。所用的处理方法为:胎牛血清(FBS)(T1)、商品鸡血清(SCK)(T2)、Pradu Hang Dam鸡血清(PDC)(T3)和乌骨鸡血清(BBC)(T4)。将乌骨鸡ES细胞在37.0°C、5%CO2的湿润环境中培养10天。通过在450nm处的吸光度测量乌骨鸡ES细胞的生长浓度。使用血细胞仪对乌骨鸡ES细胞的数量进行计数。将培养肉和鸡肉的蛋白质含量进行比较,以进行燃烧和近似分析。在完全随机设计中,使用ANOVA对所有收集的数据进行分析。T4的数量和生长率往往高于其他组,其次分别是T3、T2和T1。当最终计数ES细胞时,T4的数量和生长速率显著高于其他组(p<0.001)。比较蛋白质含量,发现培养的肉比其他组具有显著更多的蛋白质(p<001),并且特征(肌肉纤维的排列和横截面)与鸡细胞没有差异。最后,培养乌骨鸡ES细胞需要使用含有乌骨鸡血清的培养基来生长和发育用于培养肉的乌骨鸡ES细胞。
期刊介绍:
Tropical Animal Science Journal encompasses a broad range of research topics in animal sciences: breeding and genetics, reproduction and physiology, nutrition, feed sciences, agrostology, animal products, biotechnology, behaviour, welfare, health and veterinary, livestock farming system, socio-economic, animal waste management, and policy.