Comparative morphology of the musculature of the sting apparatus in Ampulex compressa (Hymenoptera, Ampulicidae) and Sceliphron destillatorium (Hymenoptera, Sphecidae)

Abstract

The sting apparatus of aculeate Hymenoptera is derived from the ovipositor and is their most prominent apomorphy. In contrast to the frequently analysed sclerites of the sting apparatus, the associated musculature has largely been neglected. In this study, we use micro-computed tomography to present a detailed description of the musculature of the sting apparatus of Ampulex compressa (Ampulicidae) and Sceliphron destillatorium (Sphecidae). We found that 12 of 15 muscles corresponding to the sting apparatus are homologous between both species examined and 13 muscles in comparison with Hymenoptera described in the literature. All muscles identified as critical for the act of stinging were found in both species. Moreover, we found the ventral tergum 8-tergum 9 muscle and the tergum 8-tergum 8 muscles in A. compressa and the second valvifer-second valvifer muscle in S. destillatorium. For the first time, we describe the ventral tergum 8-tergum 9 muscle and the second valvifer-second valvifer muscle that interconnects both body sides, in Hymenoptera.