Relationship with Metalloproteinase 13, E-cadherin, Homocysteine and Co Factors Folic Acid and Vitamin B12 in Patients Diagnosed with Prostate Cancer

Q4 Pharmacology, Toxicology and Pharmaceutics Current Enzyme Inhibition Pub Date : 2020-10-26 DOI:10.2174/1573408016999201026195354

T. Gascón, Beatriz G. Lourenço, E. Pereira, Beatriz A. da Costa Aguiar, G. L. Veiga, Flávia de Sousa Gehrke, F. Adami, F. Fonseca

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Abstract

Prostate cancer (Pc) is the most frequent neoplasia in men and the second cause of death in Brazil. To analyze the interactions and biologicals responses of Pc oxidative stress and prostatespecific antigen (PSA), E-cadherin and MMP-13. Demonstrate whether the increase of the amount of the form of E-cadherin found in the plasma of Pc patients, correlates with decrease of the PSA. Samples were obtained through peripheral venipuncture to analyse parameters of biomarkers pc as PSA, E-cadherin, MMP-13, Homocysteine, Folic acid, Vitamin B12, Testosterone T and free following the patients diagnosis, 3 and 6 months during their treatment to analyze the biological responses of Pc oxidative stress. The analysis was performed by using immunoenzymatic assay. Statistical data processed through Excel in Windows Vista and analyzed through the Shapiro-wilk Test, ANOVA, and Spearman Test. An increase in the concentration of E-cadherin (p = 0.02), as a decrease in concentration of PSA (p < 0.001), total testosterone (p < 0.001) and free testosterone (p = 0.02) was observed during the treatment period without significant alterations in the remaining markers for either of the periods. It was found that during treatment of men diagnosed with pc that there was an an increase in the concentration of plasmatic E-cadherin, which was negatively correlated with the concentrations of folic acid (-0,03 (0,87) rs (p). It was observed that the levels of hcy are positively correlated with concentrations of total testosterone and a negative correlation. Vitamins B12 remained within the parameters of normality during the entire study. P.S.A levels were free and total testosterone levels decreased. In this way, monitoring the folic acid, E-cadherin dosages of patients during the treatment phases can effectively complement in the face of remission, since it would be a way of preventing abnormal cell replications, with a clinical view prudent so that the cell methylation cycle is not affected.

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前列腺癌患者金属蛋白酶13、e -钙粘蛋白、同型半胱氨酸和钴因子、叶酸和维生素B12的关系

前列腺癌(Pc)是巴西男性中最常见的肿瘤，也是第二大死因。分析前列腺癌氧化应激与前列腺特异性抗原(PSA)、E-cadherin、MMP-13的相互作用及生物制剂反应。证明Pc患者血浆中e -钙粘蛋白形态的增加是否与PSA的降低相关。通过外周静脉穿刺取标本，分析患者诊断后、治疗3、6个月时PSA、E-cadherin、MMP-13、同型半胱氨酸、叶酸、维生素B12、睾酮T和free等生物标志物参数，分析Pc氧化应激的生物学反应。采用免疫酶法进行分析。统计数据在Windows Vista中使用Excel进行处理，并通过Shapiro-wilk检验、ANOVA和spearman检验进行分析。在治疗期间观察到E-cadherin浓度升高(p = 0.02)， PSA浓度降低(p < 0.001)，总睾酮(p < 0.001)和游离睾酮(p = 0.02)，其余标记物在任何一个时期都没有显着改变。研究发现，在诊断为pc的男性治疗期间，血浆e -钙粘蛋白浓度增加，与叶酸浓度呈负相关(- 0.03 (0.87)rs (p))。hcy水平与总睾酮浓度呈正相关，负相关(p)。在整个研究过程中，维生素B12保持在正常的参数范围内，p.s.a.水平游离，总睾酮水平下降。通过这种方式，在治疗阶段监测患者的叶酸、e -钙粘蛋白剂量可以有效地补充缓解，因为这将是一种防止异常细胞复制的方法，具有临床观点，因此细胞甲基化周期不受影响。

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来源期刊

Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

1.30

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0.00%

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期刊介绍： Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.