Mechanism of Silencing HOXC8 Increases Radiosensitivity of A549 Cells

中华放射肿瘤学杂志 Pub Date : 2019-12-15 DOI:10.3760/CMA.J.ISSN.1004-4221.2019.12.014

Rui Song, Jinjin Yuan, Ge Hou, Jun Yang, Xiaojuan Chen

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Abstract

Objective To investigate the effect and potential mechanism of HOXC8 on the radiosensitivity of non-small cell lung cancer cell line A549, aiming to provide novel ideas for clinical combined treatment. Methods The A549 cells with stable knockdown of HOXC8 were constructed by using lentivirus and validated by qPCR and Western blot. The radiosensitivity of A549 stable cell line was assessed by plate clone formation assay. The expression levels of TGF-β1 and the proteins in the downstream signal pathway after knockdown of HOXC8 were detected by Western blot. Results The A549 cells with stable knockdown of HOXC8 were successfully constructed. The viability and clonogenic capacity of A549 cells were significantly reduced after silencing HOXC8. Silencing HOXC8 also increased the sensitivity of A549 cells to radiotherapy and significantly inhibited the expression of TGF-β1 and p-Smad2/3 proteins in the downstream signaling pathway. Conclusion Silencing HOXC8 can increase the sensitivity of A549 cells to radiotherapy probably by inhibiting TGF-β1 signaling transduction. HOXC8 might play an important role in A549 cells. Key words: HOXC8 gene; A549 cell line; TGF-β1; Radiosensitivity

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HOXC8沉默提高A549细胞放射敏感性的机制

目的探讨HOXC8对非小细胞肺癌癌症细胞株A549放射敏感性的影响及其潜在机制，为临床联合治疗提供新思路。方法利用慢病毒构建稳定敲除HOXC8的A549细胞，并通过qPCR和Western印迹进行验证。用平板克隆形成法测定A549稳定细胞系的放射敏感性。通过蛋白质印迹检测HOXC8敲低后TGF-β1和下游信号通路中蛋白质的表达水平。结果成功构建了稳定敲除HOXC8的A549细胞。HOXC8沉默后A549细胞的活力和克隆能力显著降低。沉默HOXC8还增加了A549细胞对放疗的敏感性，并显著抑制下游信号通路中TGF-β1和p-Smad2/3蛋白的表达。结论HOXC8沉默可能通过抑制TGF-β1信号传导而提高A549细胞对放疗的敏感性。HOXC8可能在A549细胞中发挥重要作用。关键词：HOXC8基因；A549细胞系；TGF-β1；辐射敏感性

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来源期刊

中华放射肿瘤学杂志

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6375

期刊介绍： The Chinese Journal of Radiation Oncology is a national academic journal sponsored by the Chinese Medical Association. It was founded in 1992 and the title was written by Chen Minzhang, the former Minister of Health. Its predecessor was the Chinese Journal of Radiation Oncology, which was founded in 1987. The journal is an authoritative journal in the field of radiation oncology in my country. It focuses on clinical tumor radiotherapy, tumor radiation physics, tumor radiation biology, and thermal therapy. Its main readers are middle and senior clinical doctors and scientific researchers. It is now a monthly journal with a large 16-page format and 80 pages of text. For many years, it has adhered to the principle of combining theory with practice and combining improvement with popularization. It now has columns such as monographs, head and neck tumors (monographs), chest tumors (monographs), abdominal tumors (monographs), physics, technology, biology (monographs), reviews, and investigations and research.