Doping Agent Naphazoline Hydrochloride: Development of Simple and Fast Voltammetric Method for Its Determination in Human Serum

Abstract

Electrochemical behavior of naphazoline hydrochloride on a carbon paste electrode that was modified with aluminium oxide nanoparticles (Al 2 O 3 NPs) was investigated in a Britton-Robinson (BR) buffer (pH 7.0) using various voltammetric techniques. The results support the presence of an irreversible and diffusion-controlled electrochemical oxidation signal of naphazoline hydrochloride which is approximately at 0.9 V vs. Ag/AgCl. A selective, accurate, and simple square-wave anodic adsorptive stripping voltammetric method was purposed for naphazoline hydrochloride detection. The linear response was within the range of 5.0 × 10 -8 - 3.0 × 10 -5 mol L -1 with a detection limit of 2.6 × 10 –8 mol L –1 (0.0064 mg L –1 ). In addition, the purposed method was also utilized for naphazoline hydrochloride determination in human serum sample.