PREPARATION OF SILVER AND COPPER NANOPARTICLES IN PRESENCE OF ASCORBIC ACID AND INVESTIGATION OF THEIR ANTIBACTERIAL ACTIVITY

B. Laban, Milena Košanin, G. Isić, U. Ralević, M. Marković, A. Jokić, V. Vasić
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引用次数: 1

Abstract

In this study, we present a synthesis of silver and copper nanoparticles (NPs) using ascorbic acid as stabilizing and sodium borohydride as reducing agents, respectively. Four colloidal dispersions were obtained, two of them additionally stabilized by gelatin. They were characterized by UV-Vis, AFM, DLS and zeta potential measurements. The size of both silver and copper NPs, determined by AFM measurements, was 10 nm before, and 15 nm after stabilization with gelatin. Antibacterial activity of synthesized NPs was tested using series of gram positive and gram negative bacteria. It was found that Ag and Cu NPs showed antibacterial activity in all cases.
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抗坏血酸存在下银和铜纳米颗粒的制备及其抗菌活性的研究
在这项研究中,我们分别以抗坏血酸作为稳定剂和硼氢化钠作为还原剂合成了银和铜纳米粒子(NPs)。得到了四种胶体分散体,其中两种用明胶稳定。通过紫外-可见光谱、原子力显微镜、DLS和zeta电位测定对其进行了表征。通过原子力显微镜测定,银和铜纳米粒子在用明胶稳定前的尺寸为10 nm,在用明胶稳定后的尺寸为15 nm。采用革兰氏阳性菌和革兰氏阴性菌对合成的NPs进行抑菌活性检测。结果表明,银和铜NPs均表现出抗菌活性。
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