The isolation of halophilic urease-producing bacteria and the study of their nano-crystal production

M. Haddadi, G. Ghezelbash
{"title":"The isolation of halophilic urease-producing bacteria and the study of their nano-crystal production","authors":"M. Haddadi, G. Ghezelbash","doi":"10.29252/nbr.7.1.37","DOIUrl":null,"url":null,"abstract":". Urease-producing bacteria can precipitate calcite nano-crystals by producing urease in the presence of urea and calcium. Calcite precipitation resulting from microbial activity is a process which causes cementation of soil particles in nature. The purpose of this study was to isolate urease - producing halophilic bacteria in order to precipitate calcite in saline soil. Natural samples, including soil and saline waters, were selected for this purpose. At First, halophilic bacteria were isolated by salt-containing TSB medium. Then, a selective medium containing phenol red and urea facilitated the isolation of urease-producing bacteria . Hydrolysis of urea by urease causes alkalization of the medium and the formation of pink halo around colonies. Finally, the best isolate was selected for further study by measuring the release of ammonium by the Nessler method. The ability or inability of isolates to produce calcite was investigated by culturing the isolates on sedimentary medium with different salt concentrations for 10 days. In total, 110 halophilic isolates were isolated, among which 58 isolates had the ability of urease production. The microscopic studies of colonies showed that only 6 isolates were able to produce crystals on precipitation medium. Isolate 6 was selected for further study and then analyzed by X-ray diffraction crystals on precipitation medium. Isolate 6 was selected for further study and then analyzed by X-ray diffraction (XRD)","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"yfthhy nwyn dr `lwm zysty","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29252/nbr.7.1.37","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

. Urease-producing bacteria can precipitate calcite nano-crystals by producing urease in the presence of urea and calcium. Calcite precipitation resulting from microbial activity is a process which causes cementation of soil particles in nature. The purpose of this study was to isolate urease - producing halophilic bacteria in order to precipitate calcite in saline soil. Natural samples, including soil and saline waters, were selected for this purpose. At First, halophilic bacteria were isolated by salt-containing TSB medium. Then, a selective medium containing phenol red and urea facilitated the isolation of urease-producing bacteria . Hydrolysis of urea by urease causes alkalization of the medium and the formation of pink halo around colonies. Finally, the best isolate was selected for further study by measuring the release of ammonium by the Nessler method. The ability or inability of isolates to produce calcite was investigated by culturing the isolates on sedimentary medium with different salt concentrations for 10 days. In total, 110 halophilic isolates were isolated, among which 58 isolates had the ability of urease production. The microscopic studies of colonies showed that only 6 isolates were able to produce crystals on precipitation medium. Isolate 6 was selected for further study and then analyzed by X-ray diffraction crystals on precipitation medium. Isolate 6 was selected for further study and then analyzed by X-ray diffraction (XRD)
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
嗜盐产脂细菌的分离及其纳米晶体生产研究
. 产脲酶细菌可以在尿素和钙存在的情况下通过产生脲酶沉淀方解石纳米晶体。在自然界中,微生物活动引起的方解石沉淀是导致土壤颗粒胶结的过程。本研究的目的是分离产生脲酶的嗜盐细菌,以便在盐碱地中沉淀方解石。自然样本,包括土壤和盐水,被选择用于这个目的。首先,用含盐的TSB培养基分离嗜盐菌。然后,含有酚红和尿素的选择性培养基促进了产酶细菌的分离。脲酶对尿素的水解使培养基碱化,菌落周围形成粉红色的晕。最后,通过内斯勒法测定铵的释放量,筛选出最佳分离物进行进一步研究。通过在不同盐浓度的沉积培养基上培养10天,考察了菌株产生方解石的能力。共分离到110株嗜盐菌,其中具有产脲酶能力的有58株。菌落的显微研究表明,只有6株菌株能在沉淀培养基上结晶体。选择分离物6进行进一步研究,然后在沉淀介质上进行x射线衍射晶体分析。选择分离物6进行进一步研究,然后进行x射线衍射(XRD)分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
审稿时长
12 weeks
期刊最新文献
Green extracellular synthesis of the Fe2O3 nanoparticles by a native marine bacterium, Alcaligenes sp. strain NV06 The impact of drought stress on antioxidant activities of basil (Ocimum basilicum) cultivars extracts Study of morphological, anatomical characteristics and metabolic assay of some Ulva species (sea lettuce) in the coastal waters of the Persian Gulf, Iran Evaluating the effects of metformin on irisin and serum biochemical parameters in male sprague-dawley rats fed by a high-fat emulsion diet The effect of rhizospheric bacteria on the physiological and biochemical characteristics of safflower (Carthamus tinctorius)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1