The influence of environmental stressful conditions on the interaction between Heat Shock Proteins and chaperone-assisted proteins in land snails, Helix pomatia L.

IF 1 4区 生物学 Q3 ZOOLOGY Canadian Journal of Zoology Pub Date : 2023-08-28 DOI:10.1139/cjz-2023-0118
Paulina Agata Idczak-Figiel, M. Ostrowski, A. Nowakowska
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Abstract

Snails are often exposed to high variability of ambient temperatures, thus in response to that, they function for prolonged periods in a dormant state. It is known that molecular chaperones (like HSPs) fulfill important functions in maintaining cell homeostasis and the cellular responses to stress and that they are activated in many different species upon exposition to various environmental stressors. HSPs defend organisms from the harmful consequences of heat shock and potentially alternative stressors too. After thorough consideration, we decided to identify proteins that interact with HSP70 and HSP90 in Helix pomatia Linnaeus, 1758 snails under extreme thermal (low and high) and photoperiod (short and long) conditions and at hypometabolic/active states as a response to environmental stress. Identification of proteins that interact with HSPs can define a new tool in molecular basis of adaptation to temperature stress in snails. After performing Co-immunoprecipitation and Western blot we obtained results showing that HSP70 interacts with hemocyanin alphaN and alphaD, and with other isoforms of hemocyanin - hemocyanin beta as well as with Na+/K+-ATPase, whereas HSP90 interacts with hemocyanin alphaN. It means that the chaperones are likely to affect the most important life-supporting systems of snails like respiration and ionic conductivity.
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环境胁迫条件对钉螺热休克蛋白与伴侣辅助蛋白相互作用的影响。
蜗牛经常暴露在高度变化的环境温度中,因此,为了应对这种变化,它们在休眠状态下长时间工作。众所周知,分子伴侣(如热休克蛋白)在维持细胞稳态和细胞对应激的反应中起着重要的作用,并且在许多不同的物种中,当暴露于各种环境应激源时,它们会被激活。热休克蛋白保护生物体免受热休克和潜在替代应激源的有害后果。经过充分考虑,我们决定在Helix pomatia Linnaeus, 1758蜗牛在极端热(低和高)和光周期(短和长)条件下以及低代谢/活性状态下识别与HSP70和HSP90相互作用的蛋白,作为对环境胁迫的响应。鉴定与热休克蛋白相互作用的蛋白可以为研究蜗牛适应温度胁迫的分子基础提供新的工具。通过免疫共沉淀和Western blot,我们得到的结果显示,HSP70与血青素α和α had,以及血青素-血青素β和Na+/K+- atp酶的其他异构体相互作用,而HSP90与血青素α相互作用。这意味着伴侣可能会影响蜗牛最重要的生命支持系统,如呼吸和离子电导率。
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来源期刊
Canadian Journal of Zoology
Canadian Journal of Zoology 生物-动物学
CiteScore
2.40
自引率
0.00%
发文量
82
审稿时长
3 months
期刊介绍: Published since 1929, the Canadian Journal of Zoology is a monthly journal that reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
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