Purification and Kinetic Characterization of Hepatic Catalase from Carnivore Fish, Channa striata Exposed to Agrochemicals (endosulfan+deltamethrin)

Abstract

| From last few years contamination of aquatic bodies has been increased due to extensive use of pesticides in agriculture. The exposure of aquatic animals to these pesticides results in acute and chronic health effects. Fresh water fish species such as Channa striata serve as important bio-indicators for aquatic contamination to access the changes caused by human activities effectively and reliable monitoring bio-system to recognize and predict hazardous effects of pollutants. Therefore, this study was done to evaluate the toxicity of endosulfan (END), deltamethrin (DM) mixture on hepatic catalase (CAT) activity of fish, Channa striata exposed to the sub-lethal concentration (1/3rd of LC50) for 14 days. The CAT was partially purified by using ammonium sulphate precipitation technique. Results of partial purification demonstrated that the exposure of insecticides mixture decreased the hepatic CAT activity in fish (43.33±1.41 UmL-1) as compare to control (50.33±1.41 Unit mL-1). After desalting specific activity of hepatic CAT remain lowest in exposed fish as 134.30±0.71 Unit mg-1in relation to control (148.02±0.41 Umg-1). The fold purification of hepatic CAT from control and exposed C. striata was noted as 2.35±0.01 and 2.22±0.01, respectively. The percentage recovery of hepatic CAT from control and exposed C. striata was calculated as 81.77±1.41and 69.94±0.71, respectively. Results of kinetic characterization showed that optimum pH and temperature for hepatic CAT was noted as 7.5 and 25oC, respectively. It was concluded that the insecticides mixture exposure can alter the activity of antioxidant enzymes in fish. T hese parameters can be used as a good biomarker to evaluate the aquatic pollution. Article History Received: September 08, 2018 Revised: March 12, 2019 Accepted: May 05, 2019 Published: June 21, 2019 Authors’ Contributions FT performed the research and wrote the article. SA planned and supervised the study. HN helped in statistical anlyses. KA provided lab facilities for research. LS helped in writing the article.