Xuemei Liu, Xinyi Liao, Xiyun Rao, Bin Wang, Jun Zhang, Ge Xu, Xuejun Jiang, Xia Qin, Chengzhi Chen, Zhen Zou
{"title":"The lysosomal membrane protein LAMP‐2 is dispensable for PINK1/Parkin‐mediated mitophagy","authors":"Xuemei Liu, Xinyi Liao, Xiyun Rao, Bin Wang, Jun Zhang, Ge Xu, Xuejun Jiang, Xia Qin, Chengzhi Chen, Zhen Zou","doi":"10.1002/1873-3468.13663","DOIUrl":null,"url":null,"abstract":"Selective autophagy for the elimination of aberrant mitochondria, termed mitophagy, can be regulated by the kinase PINK1 and the ubiquitin ligase Parkin. The lysosome‐associated membrane protein 2 (LAMP‐2) plays diverse functions in non‐selective autophagy, chaperone‐mediated autophagy and selective autophagy for the degradation of RNA/DNA. In the present study, we investigated whether LAMP‐2 plays important roles during PINK1/Parkin‐mediated mitophagy. The results obtained clearly show that knockdown of LAMP‐2 does not cause defects in mitophagy in HeLa cells stably expressing Parkin, indicating that LAMP‐2 is dispensable for PINK1/Parkin‐mediated mitophagy. The present study is the first to determine the potential role of LAMP‐2 in PINK1/Parkin‐mediated mitophagy, thereby providing more insight into the sophisticated process of mitophagy.","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1873-3468.13663","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEBS Letters","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/1873-3468.13663","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 5
Abstract
Selective autophagy for the elimination of aberrant mitochondria, termed mitophagy, can be regulated by the kinase PINK1 and the ubiquitin ligase Parkin. The lysosome‐associated membrane protein 2 (LAMP‐2) plays diverse functions in non‐selective autophagy, chaperone‐mediated autophagy and selective autophagy for the degradation of RNA/DNA. In the present study, we investigated whether LAMP‐2 plays important roles during PINK1/Parkin‐mediated mitophagy. The results obtained clearly show that knockdown of LAMP‐2 does not cause defects in mitophagy in HeLa cells stably expressing Parkin, indicating that LAMP‐2 is dispensable for PINK1/Parkin‐mediated mitophagy. The present study is the first to determine the potential role of LAMP‐2 in PINK1/Parkin‐mediated mitophagy, thereby providing more insight into the sophisticated process of mitophagy.
期刊介绍：
FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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