{"title":"Hollow fibre membrane bioreactor functionalized with GO-cryogel 3D matrix promotes liver cell anchoring and their functional maintenance","authors":"Surendra Kumar Verma, Akshay Modi, Jayesh Bellare","doi":"10.1002/mds3.10128","DOIUrl":null,"url":null,"abstract":"<p>The liver support system (bioartificial liver, BAL) is a potentially efficient treatment during reversible acute liver disease or while waiting for a liver transplant. The main challenge in implementing BAL is a bioreactor, which can fulfil the needs of hepatocyte culture, mass transfer and immunobarriers. In the present study, polymer nanocomposite hollow fibre membranes (HFMs) were fabricated using polyethersulphone (P) as a base polymer, vitamin E TPGS (T) as an additive and carboxylated carbon nanotubes (C) as a filler. These PTC HFMs were functionalized with cryogel (Cr), with or without carboxylated graphene oxide (G), known as PTC-Cr or PTC-CrG HFMs, respectively. These HFM substrates were characterized and evaluated for hemocompatibility, cytotoxicity and cellular functionality. Among all the HFM substrates, PTC-CrG HFMs showed superior hemocompatibility and minimal cytotoxicity. Furthermore, a liver cell bioreactor was developed and studied using PTC-CrG HFMs. The goat primary liver cells were seeded in a bioreactor and cultured in continuous mode. The cell functionality was better maintained in the continuous mode as compared to that in the batch culture. The three-dimensional PTC-CrG HFMs-based bioreactor can be used for liver cell bioreactor, bioartificial liver system, drug testing/toxicology metabolic study and mass cell culture.</p>","PeriodicalId":87324,"journal":{"name":"Medical devices & sensors","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/mds3.10128","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical devices & sensors","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mds3.10128","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
The liver support system (bioartificial liver, BAL) is a potentially efficient treatment during reversible acute liver disease or while waiting for a liver transplant. The main challenge in implementing BAL is a bioreactor, which can fulfil the needs of hepatocyte culture, mass transfer and immunobarriers. In the present study, polymer nanocomposite hollow fibre membranes (HFMs) were fabricated using polyethersulphone (P) as a base polymer, vitamin E TPGS (T) as an additive and carboxylated carbon nanotubes (C) as a filler. These PTC HFMs were functionalized with cryogel (Cr), with or without carboxylated graphene oxide (G), known as PTC-Cr or PTC-CrG HFMs, respectively. These HFM substrates were characterized and evaluated for hemocompatibility, cytotoxicity and cellular functionality. Among all the HFM substrates, PTC-CrG HFMs showed superior hemocompatibility and minimal cytotoxicity. Furthermore, a liver cell bioreactor was developed and studied using PTC-CrG HFMs. The goat primary liver cells were seeded in a bioreactor and cultured in continuous mode. The cell functionality was better maintained in the continuous mode as compared to that in the batch culture. The three-dimensional PTC-CrG HFMs-based bioreactor can be used for liver cell bioreactor, bioartificial liver system, drug testing/toxicology metabolic study and mass cell culture.