Personalized responsiveness of human PBMCs to the action of IL-7

Abstract

Interleukin-7 (IL-7) is an important element in the functioning of the immune system. Therefore, it would be appropriate to assess individual responses of the immune cells to IL-7. Aim. To analyze ex vivo responses of the healthy adult individual T cells to increasing rhIL-7 concentrations. Methods. Isolation and cultivation of PBMCs and in vitro cytokine bioassay. Quantitative determination of cell viability was performed by the metabolic MTT-uptake assay in viable cells. EC 50 was calculated by means of OriginPro7,5. Results. The dose-response effect on T cell subsets of PBMCs for different concentrations of rhIL-7 in individuals in vitro was evaluated. IL-7 bioassay revealed that the individual EC 50 values ranged from 0.41 to 1.5 ng/ml. Inter-individual variability was high for EC 50 values (CV G =38 %). Maximal percentage increase in cell viability among subjects demonstrated moderate variability (CV G =15 %). Conclusions. The study revealed that responsiveness to IL-7 is various for the PBMCs originated from different individuals. Our research assumes that the personalized responsiveness of human PBMCs to IL-7 may be considered as valuable prognostic information about the immune system state and the T cells response to IL-7 - based therapies. The advantage of the optimized bioassay for the detection sensitivity of PBMC response to IL-7 was shown.