Optimasi Proses Ekstraksi Bunga Telang (Clitoria ternatea) Berbantu Gelombang Mikro Menggunakan Aplikasi Response Surface Methodology

Rini Azharini, A. Widyasanti, Siti Nurhasanah
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Abstract

Butterfly pea (Clitoria ternatea) had a variety of active compounds that was the potential an alternative source of natural antioxidants. Procedures for isolating active compounds could been through microwave-assisted extraction. The purpose of this research was to found out the combination of solvent volume treatment, extraction time, and microwave power that produces optimum yield and analysis of extract quality characteristics. Microwave-assisted extraction process used 10 g of butterfly pea powder with a solvent volume of 150 ml to 250 ml, extraction time of 3 minutes to 7 minutes, and power by 30% to 70%. This study used the experimental methods and optimization processes with The Respon Surface Methodology (RSM) type Box-Behnken Design (BBD). Optimum conditions of the extraction process were obtained with a solvent volume of 196 ml for 3 minutes, and microwave power of 30%.  The yield optimal response result was obtained by 53.39%, residual ethanol was 30.17%, total flavonoid compounds was 60.77 mgQE/g, specific gravity 1.082, pH 4.33 and color analysis L* 14.93, a* 0.14, b* -0.56, chromatic 0.56, hue 284.63 (Blue Purple), and TCD was in very different categories. This study analyzed that microwave-assisted extraction with variable solvent volume, time, and power affects the yield and quality characteristics of the extracts.
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蝶豆(Clitoria ternatea)含有多种活性化合物,是潜在的天然抗氧化剂的替代来源。有效成分的分离可采用微波辅助萃取法。本研究的目的是找出溶剂量处理、提取时间和微波功率的最佳产率组合,并对提取液的质量特性进行分析。微波辅助提取工艺采用蝶豆粉10 g,溶剂体积150 ~ 250 ml,提取时间3分钟~ 7分钟,功率30% ~ 70%。本研究采用响应面法(RSM)型Box-Behnken设计(BBD)的实验方法和优化过程。最佳提取工艺条件为:溶剂体积为196 ml,微波功率为30%,提取时间为3分钟。最佳响应结果为产率53.39%,乙醇残留量30.17%,总黄酮含量60.77 mgQE/g,比重1.082,pH 4.33,颜色分析L* 14.93, a* 0.14, b* -0.56,色度0.56,色相284.63(蓝紫色),与TCD相差很大。本研究分析了不同溶剂体积、时间和功率的微波辅助提取对提取物收率和质量特性的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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12 weeks
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