Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

IF 1.2 Q3 MULTIDISCIPLINARY SCIENCES The EuroBiotech Journal Pub Date : 2021-06-01 DOI:10.2478/ebtj-2021-0018

G. Tuncel, M. C. Ergoren, B. Baddal, P. Tulay, A. Arıkan, Emrah Guler, Cenk Serhan Ozverel, H. Kaya Suer, M. Sayan, T. Şanlıdağ

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Abstract

Abstract A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2. This study investigated the compatibility of two different RT-qPCR methodologies Diagnovital® and Bio-Speedy® by re-analyzing the previously confirmed positive samples. A total of 43 previously confirmed positive samples were re-analyzed by two different commercially available SARS-CoV-2 RT-qPCR kits. Only 23.5% of positive samples detected by Diagnovital® RT-qPCR kit were detected by Bio-Speedy® detection kit. In conclusion, adoption of Diagnovital® RT-qPCR kit detecting two regions of SARS-CoV-2 genome in our laboratories enabled the detection of SARS-CoV-2 in asymptomatic cases with higher sensitivity and contributed to the prevention of viral transmission within the country. The timely detection of infection in asymptomatic individuals may be the key to a successful fight against the COVID- 19 pandemic.

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新冠肺炎大流行期间无症状个体中SARS-CoV-2不同基因靶点的RT-qPCR结果比较

摘要逆转录酶定量聚合酶链式反应（RT-qPCR）被认为是最敏感的方法，目前正在用于对所有前往北塞浦路斯的入境乘客进行严重急性呼吸系统综合征冠状病毒2型筛查。本研究通过重新分析先前确认的阳性样本，调查了两种不同的RT-qPCR方法Diagnovital®和Bio-Speedy®的兼容性。通过两种不同的市售严重急性呼吸系统综合征冠状病毒2型RT-qPCR试剂盒对总共43份先前确认的阳性样本进行了重新分析。Diagnovital®RT-qPCR试剂盒检测到的阳性样本中，只有23.5%被Bio-Speedy®检测试剂盒检测出。总之，我们实验室采用Diagnovital®RT-qPCR试剂盒检测严重急性呼吸系统综合征冠状病毒2型基因组的两个区域，能够在无症状病例中以更高的灵敏度检测严重急性呼吸道综合征冠状病毒，并有助于预防病毒在国内传播。及时发现无症状个体的感染可能是成功抗击COVID-19大流行的关键。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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