Kandungan Total Fenolik dan Nilai Sun Protection Factor Ekstrak Sargassum sp.

Dimas Dharmawan, N. A. Putriana, S. Anggraeni
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Abstract

Seaweed or macroalgae is one of the natural products with biological activities such as antioxidant, antibacterial, and immunostimulant. One of them is the brown alga Sargassum sp. This brown alga produces secondary metabolites such as flavonoids and their derivatives as well as polyphenols. These compounds have many roles in counteracting ultraviolet radiation and have the potential to be developed as active ingredients of sunscreen. This study aimed to determine the total phenolic content and the Sun Protection Factor (SPF) value of Sargassum sp. originating from Pameungpeuk beach, Garut Regency. The SPF value is used to categorize how efficiently a material prevents exposure to ultraviolet radiation. Total phenolic content was measured using Follin Ciocalteu reagent with gallic acid standard. The Sargassum sp extract sample contained total phenolic of 149,04±5,14 mg GAE/g. The SPF value of Sargassum sp extract was calculated using the Mansur equation at a wavelength of 290-320 nm. The obtained SPF value of extract was 33,2±3,11 which equals to oxybenzone. The value indicated an ultra-protection capability and the potency of extract for further development as UV filter.   Rumput laut atau makroalga merupakan salah satu bahan alam dengan aktivitas biologis seperti antioksidan, aktivitas antibakteri dan imunostimulan. Salah satunya adalah alga cokelat  jenis Sargassum sp. Alga cokelat ini menghasilkan metabolit sekunder seperti  flavanoid  dan turunannya serta polifenol dan turunannya. Senyawa-senyawa tersebut banyak berperan dalam menangkal radiasi ultraviolet dan berpotensi dikembangkan sebagai bahan aktif tabir surya. Penelitian ini bertujuan untuk mengetahui kandungan total fenolik dan nilai Sun Protection Factor (SPF) dari Sargassum sp yang berasal dari pantai Pameungpeuk, Kabupaten Garut. Kandungan total fenolik diukur dengan menggunakan reagen Follin Ciocalteu dan menggunakan standar baku asam galat. Sampel ekstrak Sargassum sp mengandung total fenolik sebesar 149,04±5,14 mg GAE/g. Nilai SPF digunakan untuk mengkategorikan seberapa efisien sebuah bahan dalam menangkal paparan radiasi sinar ultraviolet. Nilai SPF ekstrak Sargassum sp dihitung menggunakan menggunakan persamaan Mansur pada panjang gelombang 290-320 nm. Nilai SPF yang didapatkan yaitu 33,2±3,11 yang tergolong ke dalam kategori ultra.
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海藻是一种具有抗氧化、抗菌、免疫刺激等生物活性的天然产物。其中一种是褐藻马尾藻。这种褐藻产生次生代谢产物,如类黄酮及其衍生物以及多酚。这些化合物在对抗紫外线辐射方面有许多作用,并有可能被开发成防晒霜的活性成分。本研究旨在测定产自Garut Regency Pameungpeuk海滩的马尾藻的总酚含量和防晒系数(SPF)值。SPF值用于对材料防止紫外线辐射的有效性进行分类。用Follin-Ciocalteu试剂和没食子酸标准品测定总酚含量。马尾藻提取物样品中总酚含量为149,04±5,14 mg GAE/g。使用Mansur方程在290-320nm的波长下计算马尾藻提取物的SPF值。提取物的SPF值为33.2±3.11,相当于氧苯酮。该值表明提取物具有超强的保护能力和作为紫外线过滤器进一步开发的效力。海藻是一种具有抗氧化、抗菌、免疫刺激等生物活性的天然物质。一种是马尾藻属的巧克力藻。这种巧克力藻产生次级代谢产物,如黄烷类及其衍生物和多酚及其衍生物。它们在维持紫外线辐射方面发挥着重要作用,并有可能发展成为活跃的太阳耀斑。本研究旨在测定Garut Cappaten Pameungpeuk海滩马尾藻的总酚含量和防晒系数(SPF)值。总酚含量使用Follin-Ciocalteu试剂和默认酸误差标准进行测量。马尾藻提取物样品中总酚含量为149.04±5.14mg GAE/g。SPF值用于分类一种物质在保持紫外线辐射显示方面的有效性。使用Mansur方程在290-320 nm波长下计算出的马尾藻SPF值。SPF值为33.2±3.11,属于超类。
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