Spatial distribution of starch, proteins and lipids in maize endosperm probed by scanning transmission X-ray microscopy

Q3 Chemistry Journal of Spectral Imaging Pub Date : 2019-11-13 DOI:10.1255/jsi.2019.a21
C. Rivard, B. Bakan, C. Boulogne, K. Elmorjani, S. Swaraj, R. Belkhou, D. Marion
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引用次数: 1

Abstract

The main storage components of the maize endosperm are starch, proteins and lipids. Starch and proteins are heterogeneously deposited, leading to the formation of vitreous and floury regions at the periphery and at the centre of the endosperm. The vitreous/floury mass ratio is a key physical parameter of maize end-uses for the food, feed and non-food sectors, as well as for the resistance of seeds to environmental aggressions. To improve maize breeding for vitreousness, one of the main issues is to finely delineate the molecular and physicochemical mechanisms associated with the formation of endosperm texture. In this context, we use scanning transmission X-ray microscopy at the C K-edge on maize endosperm resin-embedded ultrathin sections. The combination of local near edge X-ray absorption fine structure (NEXAFS) spectroscopy and high-resolution images enable us to achieve a quantitative fine description of the spatial distribution of the main components within the endosperm.
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扫描透射X射线显微术探测玉米胚乳中淀粉、蛋白质和脂质的空间分布
玉米胚乳的主要贮藏成分是淀粉、蛋白质和脂质。淀粉和蛋白质的沉积是不均匀的,导致在胚乳的外围和中心形成玻璃体和面粉区。玻璃体/面粉质量比是玉米在食品、饲料和非食品部门的最终用途以及种子对环境侵害的抵抗力的关键物理参数。为了提高玉米的玻璃性育种,一个主要的问题是精细地描述与胚乳结构形成相关的分子和物理化学机制。在这种情况下,我们使用扫描透射x射线显微镜在C - k边缘对玉米胚乳树脂包埋超薄切片。局部近边x射线吸收精细结构(NEXAFS)光谱和高分辨率图像的结合使我们能够对胚乳内主要成分的空间分布进行定量精细描述。
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来源期刊
Journal of Spectral Imaging
Journal of Spectral Imaging Chemistry-Analytical Chemistry
CiteScore
3.90
自引率
0.00%
发文量
11
审稿时长
22 weeks
期刊介绍: JSI—Journal of Spectral Imaging is the first journal to bring together current research from the diverse research areas of spectral, hyperspectral and chemical imaging as well as related areas such as remote sensing, chemometrics, data mining and data handling for spectral image data. We believe all those working in Spectral Imaging can benefit from the knowledge of others even in widely different fields. We welcome original research papers, letters, review articles, tutorial papers, short communications and technical notes.
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