Effect of substratum types on the growth of assimilators and stolons of Caulerpa okamurae (Bryopsidales, Chlorophyta)

Abstract

To examine the effects of substratum types on the growth of Caulerpa okamurae, sand surface and sand burial experiments were conducted. Five assimilators (erect fronds) per replicate were cultured for 15 d on the surface of three different treatments: fine sand (200 μm), coarse sand (600 μm), and no sand (control). Also, three stolons and three assimilators were buried by fine grain or coarse grain sands and incubated for 15 d. In both experiments, other culture conditions included 25°C, 30 μmol photons m-2 s-1, and 16 : 8 h L : D (light : dark). In both experiments, stolon + assimilator-, assimilator-, and stolon-weights were measured. Relative growth rates (RGRs) of stolon + assimilator weights ranged from 0.43 to 1.95% d-1 at no sand and fine sand treatment, respectively. RGRs for the weight of stolon + assimilator and new assimilators were significantly greater on the fine- and coarse sand surface than the control. In the burial experiments, RGRs of stolons (4.28% d-1 at coarse sand and 5.57% d-1 at fine sand, respectively) were significantly greater than those of assimilators (1.38% d-1 at fine sand and 1.82% d-1 at coarse sand, respectively). When stolons were buried, RGRs for assimilators were greater at the fine sand than at the coarse sand treatment. On the other hand, RGRs of buried assimilators for total frond weights and for newly produced stolons were significantly greater at the coarse sands than at the fine sands. In conclusion, C. okamurae grew well with all substrates of sands and showed better growth on fine sands than coarse ones. This result suggests that the growth of stolons and assimilators of C. okamurae is stimulated after stable attachment to the sand substrates by rhizophores. In addition, stolons showed higher growth rates than the assimilators in the sand burial states, indicating that stolons are more tolerant to low light than assimilators of C. okamurae.