Daya Saing Kawin Nyamuk Jantan Steril (Culex quinquefasciatus) Skala Laboratorium: Studi Awal Penggunaan Teknik Serangga Mandul dalam Pengendalian Vektor Filariasis Limfatik di Kota Pekalongan

Tri Ramadhani, Upik Kusuma Hadi, Susi Soviana, Z. Irawati, S. Sunaryo
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引用次数: 1

Abstract

Culex quinquefasciatus is the main vector of limfatic filariasis in Pekalongan City. Sterile Insect Tehnique could be an alternative vector control efforts to eliminate filariasis. The success of this technique is depend on the ability of laboratory-reared sterile males with the wild-type females. Indicator of SIT Aplication is determined by the value of the mating competitiveness and sterility to Culex quinquefasciatus (Diptera:Culicidae). The design of the research is an experimental. Gamma irradiation on the pupae (age . 15 hours) with the  doses of 0 Gy, 60 Gy, 65 Gy,70 Gy, 75 Gy and 80 Gy in BATAN Jakarta.  Male mosquitoes which emerged from the pupa then matting with a normal female. This research observed the mean  of females laying eggs ,fecundity, fertility and  mating competitiveness. This experimental research was conducted in the laboratory and the data were analyzed by ANOVA.The result showed that irradiation at the trial doses had an effect on fertility of Culex quinquefasciatus, but not  had significant effect on  fecundity and mating competitiveness . A dose of 70 Gy is the optimum dose with a fertility rate of 1.8% (sterility 98.2%) and C indexs 0,568 can be recommended for futher  semi field assays. The number of sterile males were six times compared with the wild population to increase the chances of  mating with wild-type females.
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无菌雄蚊子的交配能力(Culex quinquefasciatus)在实验室规模:在庇加兰市对不育昆虫传播媒介控制的研究
致倦库蚊是贝卡隆安市主要的丝虫病媒介。无菌虫Tehnique可能是消灭丝虫病的另一种媒介控制措施。这项技术的成功取决于实验室饲养的不育雄性和野生型雌性的能力。SIT应用的指标是由对致倦库蚊的交配竞争力和不育性的值决定的。研究的设计是一个实验。在雅加达巴丹对15小时龄的蛹进行0 Gy、60 Gy、65 Gy、70 Gy、75 Gy和80 Gy剂量的γ射线照射。从蛹中出来的雄蚊与一只正常的雌蚊交配。本研究观察了雌性的平均产卵量、繁殖力、生育能力和交配竞争力。本实验在实验室进行,并对数据进行方差分析。结果表明,试验剂量的辐照对致倦库蚊的生育能力有影响,但对其繁殖力和交配竞争力没有显著影响。70 Gy的剂量是最佳剂量,生育率为1.8%(不育率为98.2%),C指数为0568可用于进一步的半田间测定。不育雄性的数量是野生种群的六倍,以增加与野生型雌性交配的机会。
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