Improvement of amperometric laccase biosensor using enzyme-immobilized gold nanoparticles coupling with ureasil polymer as a host matrix

IF 2.1 4区 工程技术 Q3 CHEMISTRY, INORGANIC & NUCLEAR Gold Bulletin Pub Date : 2019-05-07 DOI:10.1007/s13404-019-00255-z
T. Kavetskyy, N. Stasyuk, O. Smutok, O. Demkiv, Y. Kukhazh, N. Hoivanovych, V. Boev, V. Ilcheva, T. Petkova, M. Gonchar
{"title":"Improvement of amperometric laccase biosensor using enzyme-immobilized gold nanoparticles coupling with ureasil polymer as a host matrix","authors":"T. Kavetskyy,&nbsp;N. Stasyuk,&nbsp;O. Smutok,&nbsp;O. Demkiv,&nbsp;Y. Kukhazh,&nbsp;N. Hoivanovych,&nbsp;V. Boev,&nbsp;V. Ilcheva,&nbsp;T. Petkova,&nbsp;M. Gonchar","doi":"10.1007/s13404-019-00255-z","DOIUrl":null,"url":null,"abstract":"<p>Synthesis of gold nanoparticles (Au-NPs) was performed using method of an inorganic chemical reduction. The synthesized Au-NPs showed an intensive surface plasmon resonance band at 525?nm that is typical for corresponding Au-NPs reported in literature. The structural and morphology characterizations of the obtained Au-NPs were carried out using SEM, AFM, TEM, and X-ray spectral analysis. The possibility of usage of Au-NPs as a carrier for covalent immobilization of commercial laccase and the combination of the obtained bio-Au-NPs with ureasil polymers as host matrixes for formation of bio-nanocomposite films was studied. The prospect of using the obtained bio-nanocomposite films in biosensor technologies in order to improve the bioanalytical characteristics of sensory elements has been proved.</p>","PeriodicalId":581,"journal":{"name":"Gold Bulletin","volume":"52 2","pages":"79 - 85"},"PeriodicalIF":2.1000,"publicationDate":"2019-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13404-019-00255-z","citationCount":"16","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gold Bulletin","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13404-019-00255-z","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
引用次数: 16

Abstract

Synthesis of gold nanoparticles (Au-NPs) was performed using method of an inorganic chemical reduction. The synthesized Au-NPs showed an intensive surface plasmon resonance band at 525?nm that is typical for corresponding Au-NPs reported in literature. The structural and morphology characterizations of the obtained Au-NPs were carried out using SEM, AFM, TEM, and X-ray spectral analysis. The possibility of usage of Au-NPs as a carrier for covalent immobilization of commercial laccase and the combination of the obtained bio-Au-NPs with ureasil polymers as host matrixes for formation of bio-nanocomposite films was studied. The prospect of using the obtained bio-nanocomposite films in biosensor technologies in order to improve the bioanalytical characteristics of sensory elements has been proved.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
用酶固定金纳米颗粒偶联脲脲聚合物作为宿主基质改进安培漆酶生物传感器
采用无机化学还原法制备了金纳米粒子。合成的Au-NPs在525?nm,这是文献中报道的相应au - np的典型特征。利用扫描电镜、原子力显微镜、透射电镜和x射线光谱分析对得到的Au-NPs进行了结构和形貌表征。研究了利用Au-NPs作为共价固定化商用漆酶的载体,以及将获得的生物Au-NPs与脲醛聚合物作为宿主基质结合形成生物纳米复合膜的可能性。所获得的生物纳米复合薄膜在生物传感器技术中的应用前景广阔,可以提高传感元件的生物分析特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Gold Bulletin
Gold Bulletin Chemistry-Inorganic Chemistry
CiteScore
3.70
自引率
4.50%
发文量
21
期刊介绍: Gold Bulletin is the premier international peer reviewed journal on the latest science, technology and applications of gold. It includes papers on the latest research advances, state-of-the-art reviews, conference reports, book reviews and highlights of patents and scientific literature. Gold Bulletin does not publish manuscripts covering the snthesis of Gold nanoparticles in the presence of plant extracts or other nature-derived extracts. Gold Bulletin has been published over 40 years as a multidisciplinary journal read by chemists, physicists, engineers, metallurgists, materials scientists, biotechnologists, surface scientists, and nanotechnologists amongst others, both within industry and academia. Gold Bulletin is published in Association with the World Gold Council.
期刊最新文献
Gelation of Hydrophilic Polymer Bearing Metal-coordination Units with Au(III) Ions: Application to Synthesis of Porous Gold Time-resolved spectroscopic and SERS characterization of plasmonic optical fiber sensor using glutathione-capped gold nanourchins for detection of lead in water Ornithine-stabilised gold nanoflowers for label-free sensitive detection of Hg2+ via amalgamation Dioxepine-derived surface-capping gold nanoparticles (Dd-AuNPs) induces ROS-mediated apoptosis and cell cycle arrest in A549 human lung cancer cell line Construction of folate-targeted delivery of polymer-coated gold nanoparticles: investigation of anticancer activity and apoptosis induction in parotid gland carcinoma
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1