Protective effect of nutritional supplementation of zinc-sulfate against cisplatin-induced spermatogonial and testicular dysfunctions in adult male Sprague-Dawley rats

Q3 Medicine Endocrine and Metabolic Science Pub Date : 2022-03-31 DOI:10.1016/j.endmts.2021.100116

Sunday Aderemi Adelakun , Babatunde Ogunlade , Obinna Peter Fidelis , Olusegun Dare Omotoso

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引用次数: 8

Abstract

Zinc (Zn) has proven to play a key role in spermatogenesis. The present study focused on the nutritional supplementation of Zn against cisplatin (CP)-induced spermatogonial and testicular dysfunctions.

Thirty-two (32) mature male Sprague Dawley rats were randomized into four groups of eight (n = 8) rats each – Control group (received 2 ml of normal saline); Zn group (received 1 mg/kg body weight (bwt) of Zn sulfate); Cisplatin group treated intraperitoneally with a single dose (10 mg/kg bwt) of CP; and CP + Zn group, after induction of testicular toxicity, were treated (orally) with 1 mg/kg bwt of Zn sulfate. The procedure lasted for 8 weeks. Parameters tested include testicular histology, sperm parameters, testosterone (TT), follicle-stimulating hormone (FSH) and luteinizing hormone (LH), catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione reductase (GR), thiobarbituric acid reactive substances (TBARS).

Cisplatin significantly decreases (p < 0.05) sperm quality, testosterone, FSH and LH, epithelial cell height, tubular diameter, number of spermatogonia, spermatocytes, and spermatids with concomitant increase in thiobarbituric acid reactive substances (TBARS). Zinc supplementation reversed the toxic effect of CP on sperm characterization, hormone profiling, histological and biochemical parameters.

Zinc supplementation, therefore, ameliorates the deleterious effect of CP on cytoarchitecture of the testis, protected the seminiferous epithelium, and reduced oxidative stress thereby promoting spermatogenesis.

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营养补充硫酸锌对顺铂诱导成年雄性Sprague-Dawley大鼠精原细胞和睾丸功能障碍的保护作用

锌(Zn)已被证明在精子发生中起着关键作用。本研究的重点是营养补充锌对顺铂(CP)诱导的精原和睾丸功能障碍的影响。将32只成年雄性Sprague Dawley大鼠随机分为4组，每组8只(n = 8只)。对照组(给予生理盐水2 ml);锌组(给予1 mg/kg体重硫酸锌);顺铂组腹腔注射单剂量CP(10 mg/kg bwt);CP + 锌组在诱导睾丸毒性后，口服硫酸锌1 mg/kg bwt。整个过程持续了8 周。检测参数包括睾丸组织学、精子参数、睾酮(TT)、促卵泡激素(FSH)、促黄体生成素(LH)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、谷胱甘肽还原酶(GR)、硫代巴比妥酸活性物质(TBARS)。顺铂显著降低(p < 0.05)精子质量、睾酮、FSH和LH、上皮细胞高度、小管直径、精原细胞、精母细胞和精母细胞数量，同时硫代巴比妥酸反应性物质(TBARS)增加。锌补充剂逆转了CP对精子特征、激素谱、组织学和生化参数的毒性作用。因此，补充锌可以改善CP对睾丸细胞结构的有害影响，保护精系上皮，减少氧化应激，从而促进精子发生。

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