Investigation of the expression of P-element-induced wimpy testis-interacting RNAs in human acute myeloid leukemia

IF 0.8 Q4 GENETICS & HEREDITY Meta Gene Pub Date : 2022-02-01 DOI:10.1016/j.mgene.2021.100998

Zahra Ghaseminezhad , Mohammadreza Sharifi , Amir Bahreini , Valiollah Mehrzad

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引用次数: 2

Abstract

P-element induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are a newly known class of small non-coding RNAs with 24–31 nucleotides in length. There are more than 20,000 piRNA genes in the human genome and some of them have a role in cancer. In this study, we investigated the expression of piRNAs in acute myeloid leukemia (AML). We used publicly available small RNA sequencing data derived from the peripheral blood of patients with AML and control samples to be re-analyzed for investigation of piRNA expression pattern and then was validated via the real-time polymerase chain reaction (PCR). Differential expression analysis showed that four upregulated piRNAs and six downregulated piRNAs in AML samples, among which, the piR-32,877 and piR-33,195 were the top two upregulated piRNAs. Real-time PCR was performed to validate the increased expression of these two piRNAs in AML compared to controls. Pathway analysis was also performed. The results provide evidence that piR-32,877 and piR-33,195 may serve as a new diagnostic and prognostic biomarker in AML.

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p元素诱导的弱睾丸相互作用rna在人急性髓性白血病中的表达研究

P-element induced wimpy testis (PIWI)-interacting RNAs (piRNAs)是一类新发现的长度为24-31个核苷酸的非编码小rna。人类基因组中有超过2万个piRNA基因，其中一些与癌症有关。在这项研究中，我们研究了pirna在急性髓性白血病(AML)中的表达。我们使用来自AML患者外周血和对照样本的公开小RNA测序数据，重新分析piRNA表达模式，然后通过实时聚合酶链反应(PCR)进行验证。差异表达分析显示，AML样本中有4个上调的pirna和6个下调的pirna，其中pir - 32877和pir - 33195是上调最多的两个pirna。实时荧光定量PCR验证了与对照组相比，这两种pirna在AML中的表达增加。还进行了途径分析。结果表明，piR-32,877和pir - 33195可能作为AML的一种新的诊断和预后生物标志物。

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来源期刊

Meta Gene Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

1.10

自引率

0.00%

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期刊介绍： Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.