Acid-Free Staining Procedure to Demonstrate Nerves in Whole Vertebrate Specimens with the Differentiation of Bone and Cartilage

IF 1.5 3区 生物学 Q2 ZOOLOGY Ichthyology and Herpetology Pub Date : 2022-08-26 DOI:10.1643/b2020138
A. L. H. Esguícero, F. A. Bockmann
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引用次数: 4

Abstract

The value of clearing and staining whole organisms to study vertebrate anatomy is unquestionable. These methods have been developed for over a century leading to protocols to prepare triple-stained specimens to differentiate between bones, cartilage, and nerves. Despite their potential to advance the field of comparative anatomy, nerve-staining methods have been used by a small number of vertebrate systematists in part because of the inconsistently successful preparations. Here, we report on several modifications to the current Sudan black B protocols and propose a new acid-free protocol to differentiate among bone, cartilage, and nerves in whole small vertebrates. This method may also be used to stain solely bone and cartilage by eliminating the nerve-staining steps. The technique herein described is successful for preparing juveniles and adults (including miniatures).
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无酸染色法显示具有骨和软骨分化的完整脊椎动物标本中的神经
清除和染色整个生物体对研究脊椎动物解剖学的价值是毋庸置疑的。这些方法已经发展了一个多世纪,导致了制备三重染色标本以区分骨骼、软骨和神经的方案。尽管神经染色方法有可能在比较解剖学领域取得进展,但仍有少数脊椎动物系统学家使用神经染色方法,部分原因是这些方法的制备不一致。在这里,我们报告了对当前苏丹黑B方案的几项修改,并提出了一种新的无酸方案来区分整个小型脊椎动物的骨骼、软骨和神经。该方法也可以通过消除神经染色步骤来仅用于对骨骼和软骨进行染色。本文描述的技术成功地制备了青少年和成年人(包括微缩模型)。
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来源期刊
CiteScore
3.90
自引率
6.70%
发文量
74
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