Liquid Storage of Ram Semen: Associated Damages and Improvement

Abstract

The successful application of assisted reproductive techniques (ARTs) in ovine as in other mammal species relies on many factors among which the quality of the semen used. After collection, semen samples are generally processed for storage (liquid storage or cryoconservation) before being used for insemination or in vitro embryo production. During the liquid storage process, sperm cells are exposed to artificial conditions which lead to oxidative stress—the imbalance between pro-oxidants and antioxidants (AO), following overproduction of reactive oxygen species (ROS)—resulting in ultrastructural, biochemical and functional damages of spermatozoa. Especially, viability, motility, mitochondrial activity, membrane integrity, and acrosome integrity are reduced while morphological abnormalities, DNA fragmentation, and lipid peroxidation (LPO) are increased, affecting the fertilizing ability and subsequent early embryonic development when using standard extenders. Indeed, an optimal semen extender must not only regulate and support an environment of adequate pH and buffering capacity to protect spermatozoa from osmotic and cooling stresses, but, also prevent the generation and/or scavenge excess ROS. To improve ram semen liquid storage, several methods have been developed with the supplementation of extenders with antioxidants or antioxidant like-compounds (enzymes, amino-acids, vitamins, plant extracts), seminal plasma, sugars, fatty acids, and nanoparticles being a relevant approach. Promising results have been registered with the supplementation of extenders with these compounds, confirming they can be used to preserve ram semen quality and fertility. Therefore, the present review provides an updated overview of the damages and associated mechanisms that ram spermatozoa undergo during liquid storage. Moreover, the supplementation of extenders with different compounds as a tool to improve semen storage is also discussed as well as their efficiency to reduce and/or prevent sperm damages during storage.