Histoprotective Effect of Essential Oil from Citrus aurantifolia in Testosterone-Induced Benign Prostatic Hyperplasia Rat.

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2019-09-25 eCollection Date: 2019-01-01 DOI:10.1155/2019/3031609

Desmond O Acheampong, Isaac K Barffour, Alex Boye, Ernest A Asiamah, Francis A Armah, Christian K Adokoh, Joy F Oluyemi, Benjamin Adrah, Richard Opoku, Emmanuel Adakudugu

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Abstract

Background: Benign prostatic hyperplasia (BPH) is a common urological disorder reported among ageing men.

Objective: The study assessed histoprotective effect of lime essential oil (LEO) in a rat model of testosterone-induced benign prostatic hyperplasia (BPH) and evaluated its ability to reverse testosterone-mediated changes in the testis, kidney, and liver.

Materials and methods: Adult Sprague Dawley (aged 12 weeks, 240-390 g) male rats were intramuscularly injected with testosterone enanthate (TE) (10 mg/kg) reconstituted in olive oil for ten days to establish benign prostatic hyperplasia (serum PSA level ≥ 1.24 ng/ml) in. After confirmation of BPH (sustained serum PSA level ≥ 1.24 ng/ml), rats in all groups (LEO: 30, 100, and 300 mg/kg, po, n = 6; finasteride: 15 mg/kg, po, n = 6) except model (BPH without treatment) and sham (no BPH and no treatment) groups were treated for 21 days. At the end of treatment, rats were anesthetised and blood was collected via cardiac puncture to determine serum PSA and total antioxidant capacity (TAC) levels. The prostate gland, testis, kidney, and liver were harvested, weighed, histologically processed and stained with H&E.

Results: LEO- and finasteride-treated groups recorded lesser mean prostatic weights relative to their model group. Baseline mean serum PSA level of LEO- and finasteride-treated groups reduced significantly (p < 0.05) relative to model group. Serum TAC levels were also higher in LEO- and finasteride-treated groups relative to model group. LEO-treated groups had less thickened glandular epithelium, smaller acini, fewer prostatic secretions and more fibromuscular stroma relative to model group. LEO and finasteride treatment produced improved histomorphological characteristics of testis, kidney, and liver compared to model group.

Conclusion: By the current results, Citrus aurantifolia LEO may possess active agents that can be explored for translational medicine against BPH.

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金荷叶精油对睾丸激素诱导的良性前列腺增生大鼠的组织保护作用

背景:良性前列腺增生(BPH)是一种在老年男性中常见的泌尿系统疾病。目的研究石灰精油(LEO)在睾丸激素诱导的良性前列腺增生(BPH)大鼠模型中的组织保护作用，并评估其逆转睾丸、肾脏和肝脏中睾丸激素介导的变化的能力。材料与方法将12周龄成年雄性大鼠(240 ~ 390 g)肌肉注射橄榄油重组的烯酸睾酮(TE) (10 mg/kg) 10 d，建立良性前列腺增生(血清PSA水平≥1.24 ng/ml)。确认BPH后(持续血清PSA水平≥1.24 ng/ml)，各组大鼠(LEO: 30、100、300 mg/kg, po, n = 6;非那雄胺:15 mg/kg, po, n = 6)，除模型组(未治疗BPH组)和假手术组(未治疗BPH组)外，其余治疗21 d。治疗结束后，麻醉大鼠，穿刺取血测定血清PSA和总抗氧化能力(TAC)水平。取前列腺、睾丸、肾脏和肝脏，称重，进行组织学处理，并用H&E染色。结果LEO组和非那雄胺组的平均前列腺重量均小于模型组。LEO组和非那雄胺组基线平均血清PSA水平较模型组显著降低(p < 0.05)。LEO组和非那雄胺组的血清TAC水平也高于模型组。与模型组相比，leo处理组腺上皮增厚较少，腺泡较小，前列腺分泌物减少，纤维肌间质增多。与模型组相比，LEO和非那雄胺治疗改善了睾丸、肾脏和肝脏的组织形态学特征。结论从目前的研究结果来看，金荷叶柑橘可能具有抗BPH转化医学的活性成分。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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