Development of Biosensors for Ethanol Gas Detection

Abstract

This work developed a biosensor for the measurement of ethanol gas in the air. The biosensors were synthesized by mixing signal layer materials containing SiO2 and polyimide (PI) substrates using the enzyme Alcohol Dehydrogenase (ADH) and coenzyme Nicotinamide Adenine Dinucleotide (NAD+) as a biosensor. The electrodes were coated on biosensors by DC magnetron sputtering method for test the response performance of the developed biosensors. The ADH/NAD+ was immobilized on the Ag electrode by Glutaric dialaehyde 25 wt. % cross-linking procedure. It was found that, alcohol biosensors can be exhibited sensing ethanol gas at even low concentrations from 300 ppb to very high concentrations up to 1900 ppm, response time 3 s, recovery times 1-2 minutes and good sensitivity. The SiO2 substrate has excellent, which provides significant advantages for wearable electronic device that compact, easy to use and reduce direct contact with alcoholics. The alcohol biosensors can adoption in next generation to other electronic devices, because easy to integrate, such as a module alcohol biosensor with wireless or the fabrication of the RCL circuit. Furthermore, the alcohol biosensors based on SiO2/Ag/ADH, PI/Ag/ADH is artificial intelligence strategy for stable practical wearable electronic devices.