Extracellular self-DNA induces local inhibition of growth, regulates production of reactive oxygen species, and gene expression in rice roots

IF 0.8 4区 生物学 Q4 PLANT SCIENCES Biologia Plantarum Pub Date : 2023-01-20 DOI:10.32615/bp.2022.037
T. O. S. Tjia, K. Meitha, P. Septiani, R. Awaludin, D. Sumardi
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引用次数: 1

Abstract

The capacity of extracellular self-DNA (esDNA) to inhibit growth is getting more research attention as this could be explored for several purposes, including the development of specific bioherbicides. While the inhibitory effect has been studied in several dicotyledon species, little is known about the effects and subsequent signaling processes in monocots. Here, we measured the growth, counted the number of lateral and crown roots, determined greenness index, quantified the production of O 2.- and H 2 O 2 , and determined the expressions of genes encoding antioxidant enzymes ( SOD s and CAT s) in rice ( Oryza sativa L.), a model plant of monocots. After 7 d of germination, rice roots were exposed to 0, 75, and 150 µg cm -3 of esDNA. Inhibitory effect was found to be negatively correlated to esDNA concentration, as indicated by the length of primary roots. Interestingly, this negative effect was only observed in the directly exposed organ (root) but not in the length of shoot or fresh mass of the whole seedling. The percentage of greenness index of leaves and number of crown and lateral roots were also similar across treatments. However, esDNA exposure to root increased production of O 2.- and H 2 O 2 in the root. At the molecular level, the response was characterized by the decreased expression of the antioxidant genes SOD 3, CAT B, and CAT C. These findings suggest that esDNA inhibits rice growth locally in, e.g. in treated roots, and the responses involve increased production of ROS and suppression of antioxidants. This study could be the basis for determining the combination of concentration and period of exposure that might significantly inhibit total growth of monocot weeds with a minimum effect on the crop.
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细胞外自身DNA诱导水稻根系生长的局部抑制,调节活性氧的产生和基因表达
细胞外自身DNA(esDNA)抑制生长的能力正受到越来越多的研究关注,因为这可以用于多种目的,包括开发特定的生物除草剂。虽然已经在几种双子叶植物中研究了这种抑制作用,但对单子叶植物的抑制作用和随后的信号传导过程知之甚少。在这里,我们测量了水稻(Oryza sativa L.)的生长,计数了侧根和冠根的数量,确定了绿色指数,量化了O2和H2 O2的产生,并确定了编码抗氧化酶(SOD和CAT)的基因在单子叶植物模型植物中的表达。发芽7天后,将水稻根暴露于0、75和150µg cm-3的esDNA中。抑制作用与esDNA浓度呈负相关,如主根长度所示。有趣的是,这种负面影响只在直接暴露的器官(根)中观察到,而在整个幼苗的茎长或新鲜质量中没有观察到。不同处理的叶片绿色指数百分比、冠根和侧根数量也相似。然而,esDNA暴露于根中增加了根中O2和H2 O2的产生。在分子水平上,这种反应的特征是抗氧化基因SOD 3、CAT B和CAT C的表达减少。这些发现表明,esDNA在处理过的根中局部抑制水稻生长,并且这种反应涉及ROS的产生增加和抗氧化剂的抑制。这项研究可以作为确定浓度和暴露时间组合的基础,这些浓度和暴露期可能会显著抑制单子叶杂草的总生长,对作物的影响最小。
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来源期刊
Biologia Plantarum
Biologia Plantarum 生物-植物科学
CiteScore
2.80
自引率
0.00%
发文量
28
审稿时长
3.3 months
期刊介绍: BIOLOGIA PLANTARUM is an international journal for experimental botany. It publishes original scientific papers and brief communications, reviews on specialized topics, and book reviews in plant physiology, plant biochemistry and biophysics, physiological anatomy, ecophysiology, genetics, molecular biology, cell biology, evolution, and pathophysiology. All papers should contribute substantially to the current level of plant science and combine originality with a potential general interest. The journal focuses on model and crop plants, as well as on under-investigated species.
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