Ganoderma lucidum extract reverses hepatocellular carcinoma multidrug resistance via inhibiting the function of P-glycoprotein in vitro and in vivo

IF 3.6 4区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Italian Journal of Food Science Pub Date : 2023-08-12 DOI:10.15586/ijfs.v35i3.2354

Jing Li, Lin Cao, Cheng Yuan, Zhaojian Jiang, Hongfei Cai, Wendong Xu, Yamin Han, Liang Chen, Qin Zhang, Renwang Jiang, Juyan Liu

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Abstract

Cancer is a leading cause of death globally. Chemotherapy still plays an indispensable role in the clinical treatment of cancer. However, the emergence of multidrug resistance (MDR) has greatly obstructed the further application of chemotherapy agents. Ganoderma lucidum (G. lucidum) is a traditional Chinese medicine as well as an edible mushroom. In this study, we first explored the effect of six extract samples derived from G. lucidum on the cell viability of adriamycin-resistant human hepatocellular carcinoma multidrug-resistant cell subline (HepG-2/ADM). All these samples showed no obvious toxicity to cells; however, only G. lucidum ethanol extract could reverse resistance to doxorubicin and paclitaxel. Then the P-glycoprotein (P-gp) function in hepatocellular carcinoma G2 (HepG-2) and HepG-2/ADM cells was determined after incubated with these samples, and we found that G. lucidum ethanol extract could inhibit P-gp function in vitro. Furthermore, G. lucidum ethanol extract could reverse resistance to paclitaxel in HepG-2/ADM tumor-bearing mice in vivo, while the protein expression level of P-gp was unchanged. Taken together, these results indicated the potential role of G. lucidum ethanol extract in reversing MDR in the clinical treatment of hepatocellular carcinoma.

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灵芝提取物通过抑制体内和体外p -糖蛋白的功能逆转肝癌多药耐药

癌症是全球死亡的主要原因。化疗在癌症的临床治疗中仍然发挥着不可缺少的作用。然而，多药耐药(MDR)的出现极大地阻碍了化疗药物的进一步应用。灵芝(Ganoderma lucidum)是一种中药，也是一种食用菌。在本研究中，我们首次探讨了六种灵芝提取物样品对阿霉素耐药人肝癌多药耐药细胞亚群(HepG-2/ADM)细胞活力的影响。所有样品对细胞均无明显毒性;而只有灵芝乙醇提取物能够逆转对阿霉素和紫杉醇的耐药性。然后用这些样品孵育后测定p -糖蛋白(P-gp)在肝癌G2 (HepG-2)和HepG-2/ADM细胞中的功能，我们发现灵光乙醇提取物在体外可以抑制P-gp的功能。此外，在P-gp蛋白表达水平不变的情况下，灵芝乙醇提取物可在体内逆转HepG-2/ADM荷瘤小鼠对紫杉醇的耐药。综上所述，这些结果表明灵芝乙醇提取物在临床治疗肝癌中具有逆转多药耐药的潜在作用。

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来源期刊

Italian Journal of Food Science 工程技术-食品科技

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： "Italian Journal of Food Science" is an international journal publishing original, basic and applied papers, reviews, short communications, surveys and opinions on food science and technology with specific reference to the Mediterranean Region. Its expanded scope includes food production, food engineering, food management, food quality, shelf-life, consumer acceptance of foodstuffs, food safety and nutrition, energy and environmental aspects of food processing on the whole life cycle. Reviews and surveys on specific topics relevant to the advance of the Mediterranean food industry are particularly welcome.