Hypoglycemic activities of flowers of Xanthoceras sorbifolia and identification of anti-oxidant components by off-line UPLC-QTOF-MS/MS-free radical scavenging detection

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2024-01-01 DOI:10.1016/j.chmed.2022.11.009
Xiajing Xu , Yongli Guo , Menglin Chen , Ning Li , Yi Sun , Shumeng Ren , Jiao Xiao , Dongmei Wang , Xiaoqiu Liu , Yingni Pan
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Abstract

Objective

To identify phytochemical constituents present in the extract of flowers of Xanthoceras sorbifolia and evaluate their anti-oxidant and anti-hyperglycemic capacities.

Methods

The AlCl3 colorimetric method and Prussian Blue assay were used to determine the contents of total flavonoids and total phenolic acids in extraction layers, and the bioactive layers was screened through anti − oxidative activity in vitro. The Waters ACQUITY UPLC system and a Waters ACQUITY UPLC BEH C18 column (2.0 mm × 150 mm, 5 μm) were used to identify the ingredients. And anti-oxidative ingredients were screened by off-line UPLC-QTOF-MS/MS-free radical scavenging. The ameliorative role of it was further evaluated in a high-fat, streptozotocin-induced type 2 diabetic rat model and the study was carried out on NADPH oxidase (PDB ID: 2CDU) by molecular docking.

Results

Combined with the results of activity screening in vitro, the anti − oxidative part was identified as the ethyl acetate layer. A total of 24 chemical constituents were identified by liquid chromatography-mass spectrometry in the ethyl acetate layer and 13 main anti-oxidative active constituents were preliminarily screened out through off-line UPLC-QTOF-MS/MS-free radical scavenging. In vivo experiments showed that flowers of X. sorbifolia could significantly reduce the blood glucose level of diabetic mice and alleviate liver cell damage. Based on the results of docking analysis related to the identified phytocompounds and oxidase which involved in type 2 diabetes, quercetin 3-O-rutinoside, kaempferol-3-O-rhamnoside, isorhamnetin-3-O-glucoside, and isoquercitrin showed a better inhibitory profile.

Conclusion

The ethyl acetate layer was rich in flavonoids and phenolic acids and had significant anti-oxidant activity, which could prevent hyperglycemia. This observed activity profile suggested X. sorbifolia flowers as a promising new source of tea to develop alternative natural anti-diabetic products with a high safety margin.

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文冠花的降血糖活性及抗氧化成分的离线UPLC-QTOF-MS/MS自由基清除检测
方法采用AlCl3比色法和普鲁士蓝法测定提取物中总黄酮类化合物和总酚酸类化合物的含量,并通过体外抗氧化活性筛选生物活性层。采用 Waters ACQUITY UPLC 系统和 Waters ACQUITY UPLC BEH C18 色谱柱(2.0 mm × 150 mm, 5 μm)鉴定成分。并通过离线 UPLC-QTOF-MS/MS 无自由基清除法筛选抗氧化成分。结果结合体外活性筛选结果,确定抗氧化部分为乙酸乙酯层。通过液相色谱-质谱法鉴定了乙酸乙酯层中的 24 种化学成分,并通过离线 UPLC-QTOF-MS/MS 无自由基清除法初步筛选出 13 种主要的抗氧化活性成分。体内实验表明,山银花能显著降低糖尿病小鼠的血糖水平,减轻肝细胞损伤。根据所鉴定的植物化合物与 2 型糖尿病氧化酶的对接分析结果,槲皮素-3-O-芸香糖苷、山奈素-3-O-鼠李糖苷、异鼠李素-3-O-葡萄糖苷和异槲皮素具有较好的抑制作用。观察到的这一活性特征表明,X. sorbifolia 花是一种很有前景的新茶源,可用于开发安全系数高的天然抗糖尿病产品。
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来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
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