Identification and Expression Analysis of a cDNA Encoding Cyclophilin A from Gryllus bimaculatus (Orthoptera: Gryllidae)

Kisang Kwon, Eun-Ryeong Lee, Kyung-Hee Kang, Seung-Whan Kim, Hyewon Park, Junghae Kim, A. Lee, O. Kwon
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Abstract

Cyclophilin A (CypA), a cytosolic binding protein of cyclosporine A, is an immunosuppressive drug. In this study, CypA cDNA was cloned from the two-spotted cricket Gryllus bimaculatus (gCypA). The protein encoded by gCypA comprises 165 amino acids with a molecular mass of 19.23 kDa and an isoelectric point of 9.38 and possesses three N-glycosylation sites and 17 phosphorylation sites. The secondary and tertiary structures of gCypA were identified, and homology analysis revealed that it shares around 73%-81% sequence identities with other CypA proteins. When the researchers analyzed the expression levels of gCypA mRNA in various tissues, they found that the foregut exhibited nearly the same expression level as that of the dorsal longitudinal flight muscle (the control). However, gCypA mRNA expression in the fat body, Malpighian tubes, and midgut was less than half of that in the dorsal longitudinal flight muscle. Under endoplasmic reticulum stress conditions, gCypA mRNA expression was highest in Malpighian tubules (about two times higher than the expression in the control). Under starvation conditions, gCypA mRNA expression increased to three times that of the dorsal longitudinal flight muscle 6 days after starvation. Nonetheless, its expression levels decreased in Malpighian tubules under all starvation conditions. This study provides insights into the physiological role of gCypA in G. bimaculatus.
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双斑Gryllus bimaculatus亲环素a基因cDNA的鉴定与表达分析(直翅目:Gryllide)
亲环素A(CypA)是环孢菌素A的胞浆结合蛋白,是一种免疫抑制药物。本研究从双斑蟋蟀(gCypA)中克隆了CypA的cDNA。gCypA编码的蛋白质包含165个氨基酸,分子量为19.23kDa,等电点为9.38,具有3个N-糖基化位点和17个磷酸化位点。对gCypA的二级和三级结构进行了鉴定,同源性分析表明,它与其他CypA蛋白的序列同源性约为73%-81%。当研究人员分析gCypA mRNA在各种组织中的表达水平时,他们发现前肠的表达水平与背部纵向飞行肌(对照)几乎相同。然而,gCypA mRNA在脂肪体、马皮根管和中肠中的表达不到背部纵向飞行肌的一半。在内质网应激条件下,gCypA mRNA在马尔皮氏管中的表达最高(约为对照中表达的两倍)。在饥饿条件下,gCypA mRNA的表达在饥饿6天后增加到背侧纵向飞行肌的三倍。尽管如此,在所有饥饿条件下,其在马尔皮氏管中的表达水平都有所下降。本研究为深入了解gCypA在双斑G.bimaculatus中的生理作用提供了见解。
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来源期刊
CiteScore
1.90
自引率
0.00%
发文量
118
期刊介绍: WSEAS Transactions on Environment and Development publishes original research papers relating to the studying of environmental sciences. We aim to bring important work to a wide international audience and therefore only publish papers of exceptional scientific value that advance our understanding of these particular areas. The research presented must transcend the limits of case studies, while both experimental and theoretical studies are accepted. It is a multi-disciplinary journal and therefore its content mirrors the diverse interests and approaches of scholars involved with sustainable development, climate change, natural hazards, renewable energy systems and related areas. We also welcome scholarly contributions from officials with government agencies, international agencies, and non-governmental organizations.
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