Sulphide Donor Exhibits Cytoprotective and Antioxidative Activity in UV-induced HaCaT Cell Lines

Abstract

Oxidative stress due to excessive of reactive oxygen species (ROS) in the body is commonly associated as one of the underlying mechanisms of ultraviolet radiation (UV)-induced damage in the skin. Reactive sulphur species (RSS) has been implicated with potent antioxidant properties and involves in modulating cellular signalling transduction pathway. In this current study, the potential involvement of the RSS in alleviating UV-induced damage in HaCaT keratinocytes was explored. We first established the suitable dose of UVA and UVB radiation that can depressed HaCaT cells population, 80 mJ/cm2 and 10 mJ/cm2, respectively. The sulphide donor, NaHS, was also tested for its cytotoxicity profile, and 200 µM was considered non-toxic concentration for the cells. Our data showed that pre-treating the cells with 200 µM of NaHS decreased the detrimental effects of UV radiation especially UVA on HaCaT cells as demonstrated by the MTT assay. In this work, we used 2’,7’-dichlorofluorescin-diacetate (DCFH-DA), a useful indicator of ROS, with regard to the determining of antioxidant potential of NaHS. Our data demonstrate that 200 µM of NaHS significantly suppressed the intracellular ROS production induced by both UVA and UVB irradiation in comparison with cells absence of NaHS (p<0.05). In conclusion, supplementation of sulphide in biological condition particularly NaHS provides protective effect for skin against UV irradiation by diminishing the formation of ROS. Nonetheless, this study provides a preliminary insight on the potential role of RSS in modulating UV-induced damages in in vitro skin model which can be further expand with precision technique in the future.