{"title":"Effect of Garlic, Stinky Bean, Dogfruit, Tomato Extracts, and N-acetylcysteine on Rats after 5 Gy Irradiation","authors":"T. Kisnanto, Iin Kurnia, M. Sadikin","doi":"10.17146/aij.2020.968","DOIUrl":null,"url":null,"abstract":"The f ormation of reactive oxygen species (ROS) and free radicals is the most important effect of radiation exposure on biological system s . Several studies have shown that several vegetables are proven to have beneficial effect s to protect the body from free radical attacks. This current study was focused on exploring the capability of extracts of garlic, stinky bean, dog fruit, and tomato, as well as N-acetylcystein (NAC), in counteracting free radicals induced by gamma irradiation with a dose of 5 Gy. Seven treatments on male rat were as follow: A (control), B (5 Gy), C (garlic + 5 Gy), D (stinky bean + 5 Gy), E (dog fruit + 5 Gy), F (tomato + 5 Gy) and G (NAC + 5 Gy). The rats were irradiated 8 days after the sup p lement had been given. Detection of malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), and 8-hydroxy-2-deoxyguanosine (8-OHdG) was done by biochemical assays, and γ-H2AX foci w ere counted by immunofluorescence assay to the lymphocytes and plasma samples. The results showed that gamma irradiation with a dose of 5 Gy caused increases in the level of MDA, 8-OHdG, and γ-H2AX foci while decreases were recorded in the level of GSH, GPx, and CAT (p < 0.05). The treatment of garlic, dog fruit, and tomato extracts and NAC reduced free radicals significantly. In conclusion, the tomato has the best ability to overcome free radicals due to gamma irradiation among the treatments in the experiment. User 14.00 Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ \n table.MsoNormalTable \n {mso-style-name:\"Table Normal\"; \n mso-tstyle-rowband-size:0; \n mso-tstyle-colband-size:0; \n mso-style-noshow:yes; \n mso-style-priority:99; \n mso-style-parent:\"\"; \n mso-padding-alt:0cm 5.4pt 0cm 5.4pt; \n mso-para-margin:0cm; \n mso-para-margin-bottom:.0001pt; \n mso-pagination:widow-orphan; \n font-size:10.0pt; \n font-family:\"Times New Roman\",\"serif\"; \n mso-ansi-language:EN-US; \n mso-fareast-language:EN-US;}","PeriodicalId":8647,"journal":{"name":"Atom Indonesia","volume":" ","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2020-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Atom Indonesia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17146/aij.2020.968","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NUCLEAR SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 1
Abstract
The f ormation of reactive oxygen species (ROS) and free radicals is the most important effect of radiation exposure on biological system s . Several studies have shown that several vegetables are proven to have beneficial effect s to protect the body from free radical attacks. This current study was focused on exploring the capability of extracts of garlic, stinky bean, dog fruit, and tomato, as well as N-acetylcystein (NAC), in counteracting free radicals induced by gamma irradiation with a dose of 5 Gy. Seven treatments on male rat were as follow: A (control), B (5 Gy), C (garlic + 5 Gy), D (stinky bean + 5 Gy), E (dog fruit + 5 Gy), F (tomato + 5 Gy) and G (NAC + 5 Gy). The rats were irradiated 8 days after the sup p lement had been given. Detection of malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), and 8-hydroxy-2-deoxyguanosine (8-OHdG) was done by biochemical assays, and γ-H2AX foci w ere counted by immunofluorescence assay to the lymphocytes and plasma samples. The results showed that gamma irradiation with a dose of 5 Gy caused increases in the level of MDA, 8-OHdG, and γ-H2AX foci while decreases were recorded in the level of GSH, GPx, and CAT (p < 0.05). The treatment of garlic, dog fruit, and tomato extracts and NAC reduced free radicals significantly. In conclusion, the tomato has the best ability to overcome free radicals due to gamma irradiation among the treatments in the experiment. User 14.00 Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */
table.MsoNormalTable
{mso-style-name:"Table Normal";
mso-tstyle-rowband-size:0;
mso-tstyle-colband-size:0;
mso-style-noshow:yes;
mso-style-priority:99;
mso-style-parent:"";
mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
mso-para-margin:0cm;
mso-para-margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:10.0pt;
font-family:"Times New Roman","serif";
mso-ansi-language:EN-US;
mso-fareast-language:EN-US;}
期刊介绍：
The focus of Atom Indonesia is research and development in nuclear science and technology. The scope of this journal covers experimental and analytical research in nuclear science and technology. The topics include nuclear physics, reactor physics, radioactive waste, fuel element, radioisotopes, radiopharmacy, radiation, and neutron scattering, as well as their utilization in agriculture, industry, health, environment, energy, material science and technology, and related fields.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
