AFLP Markers for Genetic Diversity Evaluation of Pomegranate (Punica granatum L.) in Duhok Province, Kurdistan Region – Iraq

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture Pub Date : 2020-11-18 DOI:10.15835/buasvmcn-hort:2020.0055

D. Y. Sinjare, J. Jubrael

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引用次数: 1

Abstract

In this work, 15 pomegranate (Punica granatum L.) cultivars collected throughout Duhok Province in Kurdistan-Iraq were characterized using ten AFLP primer combinations. A high degree of genetic diversity was found among tested cultivars. The total scored number of amplified fragments was 435 of which 374 were polymorphic. The rate of polymorphism varied ranging 40 to 100% with an average percentage of 86.64. The UPGMA clustering analysis based on these data revealed three main groups and that cultivars were not grouped by their geographical distribution. All calculated genetic parameters including PIC, RP, EMR and MI mean results were 0.2974, 45.66, 22.79 and 6.872 respectively, further revealed that pomegranate varieties/cultivars in this region have a rich genetic diversity. The findings showed that a molecular characterization was necessary in order to have a reliable result to evaluate and determine relationships between pomegranate genotypes.

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伊拉克杜胡克省石榴(Punica granatum L.)遗传多样性评价的AFLP标记

利用10个AFLP引物组合对在伊拉克库尔德斯坦-伊拉克杜胡克省收集的15个石榴品种进行了鉴定。被试品种间存在高度的遗传多样性。扩增片段总数为435个，其中多态片段374个。多态性率为40 ~ 100%，平均为86.64%。UPGMA聚类分析表明，品种间的地理分布不一致，主要有3个类群。遗传参数PIC、RP、EMR和MI均值分别为0.2974、45.66、22.79和6.872，表明该地区石榴品种/栽培品种具有丰富的遗传多样性。研究结果表明，为了有可靠的结果来评价和确定石榴基因型之间的关系，分子表征是必要的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture

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8 weeks