Modulation of tomato root architecture and root hair traitsby Pseudomonas brassicacearum and Variovorax paradoxus containing 1-aminocyclopropane-1-carboxylate deaminase

IF 0.8 4区 生物学 Q4 PLANT SCIENCES Biologia Plantarum Pub Date : 2022-09-17 DOI:10.32615/bp.2022.025
A. Belimov, P. Ulianich, D. Syrova, A. Shaposhnikov, V. Safronova, I. Dodd
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引用次数: 2

Abstract

By decreasing root 1-aminocyclopropane-1-carboxylate (ACC) content and plant ethylene production, the microbial enzyme ACC deaminase is a widespread beneficial trait of plant growth-promoting rhizobacteria (PGPR), ameliorating ethylene-mediated root growth inhibition. However, relatively little is known about whether bacterial ACC deaminase modulates root architecture and root hair traits. Thus the dwarf tomato ( Solanum lycopersicum ) cultivar Micro-Tom was inoculated in vitro with Pseudomonas brassicacearum Am3, its ACC deaminase deficient mutant T8-1, a known PGPR strain Variovorax paradoxus 5C-2 or chemically treated with agents that promoted or inhibited ethylene production or sensitivity (Ag + , Co 2+ , and ACC). ACC treatment reduced both root elongation and the number of lateral roots, while ethylene inhibitors (Ag + , Co 2+ ) and V. paradoxus 5C-2 promoted primary root elongation, but differentially affected lateral root length and number. Ag + stimulated lateral root development, while Co 2+ and V. paradoxus 5C-2 did not. Inoculation with P. brassicacearum Am3 and T8-1 inhibited elongation of the primary and lateral roots at a high inoculum concentration (10 6 cells cm 3 ). All bacterial strains significantly increased the length and number of root hairs, with these effects more pronounced in P. brassicacearum Am3 than in the mutant T8-1. Treatment with Ag + inhibited root hair formation and elongation, while Co 2+ had the opposite effects. ACC treatment had no effect on root hair elongation but increased root hair density. While root growth inhibition caused by P. brassicacearum Am3 was independent of ACC deaminase, the promotion of root hair elongation and density by this strain was augmented by ACC deaminase activity. Thus ACC deaminase can modulate the morphological impacts of bacteria on root hair response by affecting plant ethylene content.
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含1-氨基环丙烷-1-羧酸脱氨酶的青花假单胞菌和异花椒对番茄根构和根毛性状的调控
微生物酶ACC脱氨酶通过降低根系1-氨基环丙烷-1-羧酸盐(ACC)含量和植物乙烯产量,是促进植物生长的根细菌(PGPR)的一种广泛的有益特性,可以改善乙烯介导的根系生长抑制。然而,关于细菌ACC脱氨酶是否调节根系结构和根毛性状,目前知之甚少。因此,矮化番茄(Solanum lycopersicum)栽培品种Micro Tom在体外接种假单胞菌brassicacearum Am3、其ACC脱氨酶缺陷突变体T8-1、已知的PGPR菌株Variovorax paradoxus 5C-2,或用促进或抑制乙烯产生或敏感性的试剂(Ag+、Co2+和ACC)进行化学处理。ACC处理降低了根伸长和侧根数量,而乙烯抑制剂(Ag+,Co2+)和V.paradous 5C-2促进了主根伸长,但对侧根长度和数量的影响不同。Ag+对侧根发育有促进作用,而Co2+和V.paradous 5C-2则没有。用P.brassicacearum Am3和T8-1接种在高接种浓度(10 6个细胞cm 3)下抑制主根和侧根的伸长。所有菌株都显著增加了根毛的长度和数量,其中这些影响在甘蓝型油菜Am3中比在突变体T8-1中更明显。Ag+处理抑制根毛的形成和伸长,而Co2+处理则相反。ACC处理对根毛伸长没有影响,但增加了根毛密度。虽然甘蓝型油菜Am3引起的根系生长抑制与ACC脱氨酶无关,但ACC脱氨蛋白酶活性增强了该菌株对根毛伸长和密度的促进作用。因此ACC脱氨酶可以通过影响植物乙烯含量来调节细菌对根毛反应的形态学影响。
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来源期刊
Biologia Plantarum
Biologia Plantarum 生物-植物科学
CiteScore
2.80
自引率
0.00%
发文量
28
审稿时长
3.3 months
期刊介绍: BIOLOGIA PLANTARUM is an international journal for experimental botany. It publishes original scientific papers and brief communications, reviews on specialized topics, and book reviews in plant physiology, plant biochemistry and biophysics, physiological anatomy, ecophysiology, genetics, molecular biology, cell biology, evolution, and pathophysiology. All papers should contribute substantially to the current level of plant science and combine originality with a potential general interest. The journal focuses on model and crop plants, as well as on under-investigated species.
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