Modulation of tomato root architecture and root hair traitsby Pseudomonas brassicacearum and Variovorax paradoxus containing 1-aminocyclopropane-1-carboxylate deaminase

IF 0.8 4区 生物学 Q4 PLANT SCIENCES Biologia Plantarum Pub Date : 2022-09-17 DOI:10.32615/bp.2022.025
A. Belimov, P. Ulianich, D. Syrova, A. Shaposhnikov, V. Safronova, I. Dodd
{"title":"Modulation of tomato root architecture and root hair traitsby Pseudomonas brassicacearum and Variovorax paradoxus containing 1-aminocyclopropane-1-carboxylate deaminase","authors":"A. Belimov, P. Ulianich, D. Syrova, A. Shaposhnikov, V. Safronova, I. Dodd","doi":"10.32615/bp.2022.025","DOIUrl":null,"url":null,"abstract":"By decreasing root 1-aminocyclopropane-1-carboxylate (ACC) content and plant ethylene production, the microbial enzyme ACC deaminase is a widespread beneficial trait of plant growth-promoting rhizobacteria (PGPR), ameliorating ethylene-mediated root growth inhibition. However, relatively little is known about whether bacterial ACC deaminase modulates root architecture and root hair traits. Thus the dwarf tomato ( Solanum lycopersicum ) cultivar Micro-Tom was inoculated in vitro with Pseudomonas brassicacearum Am3, its ACC deaminase deficient mutant T8-1, a known PGPR strain Variovorax paradoxus 5C-2 or chemically treated with agents that promoted or inhibited ethylene production or sensitivity (Ag + , Co 2+ , and ACC). ACC treatment reduced both root elongation and the number of lateral roots, while ethylene inhibitors (Ag + , Co 2+ ) and V. paradoxus 5C-2 promoted primary root elongation, but differentially affected lateral root length and number. Ag + stimulated lateral root development, while Co 2+ and V. paradoxus 5C-2 did not. Inoculation with P. brassicacearum Am3 and T8-1 inhibited elongation of the primary and lateral roots at a high inoculum concentration (10 6 cells cm 3 ). All bacterial strains significantly increased the length and number of root hairs, with these effects more pronounced in P. brassicacearum Am3 than in the mutant T8-1. Treatment with Ag + inhibited root hair formation and elongation, while Co 2+ had the opposite effects. ACC treatment had no effect on root hair elongation but increased root hair density. While root growth inhibition caused by P. brassicacearum Am3 was independent of ACC deaminase, the promotion of root hair elongation and density by this strain was augmented by ACC deaminase activity. Thus ACC deaminase can modulate the morphological impacts of bacteria on root hair response by affecting plant ethylene content.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biologia Plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.32615/bp.2022.025","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 2

Abstract

By decreasing root 1-aminocyclopropane-1-carboxylate (ACC) content and plant ethylene production, the microbial enzyme ACC deaminase is a widespread beneficial trait of plant growth-promoting rhizobacteria (PGPR), ameliorating ethylene-mediated root growth inhibition. However, relatively little is known about whether bacterial ACC deaminase modulates root architecture and root hair traits. Thus the dwarf tomato ( Solanum lycopersicum ) cultivar Micro-Tom was inoculated in vitro with Pseudomonas brassicacearum Am3, its ACC deaminase deficient mutant T8-1, a known PGPR strain Variovorax paradoxus 5C-2 or chemically treated with agents that promoted or inhibited ethylene production or sensitivity (Ag + , Co 2+ , and ACC). ACC treatment reduced both root elongation and the number of lateral roots, while ethylene inhibitors (Ag + , Co 2+ ) and V. paradoxus 5C-2 promoted primary root elongation, but differentially affected lateral root length and number. Ag + stimulated lateral root development, while Co 2+ and V. paradoxus 5C-2 did not. Inoculation with P. brassicacearum Am3 and T8-1 inhibited elongation of the primary and lateral roots at a high inoculum concentration (10 6 cells cm 3 ). All bacterial strains significantly increased the length and number of root hairs, with these effects more pronounced in P. brassicacearum Am3 than in the mutant T8-1. Treatment with Ag + inhibited root hair formation and elongation, while Co 2+ had the opposite effects. ACC treatment had no effect on root hair elongation but increased root hair density. While root growth inhibition caused by P. brassicacearum Am3 was independent of ACC deaminase, the promotion of root hair elongation and density by this strain was augmented by ACC deaminase activity. Thus ACC deaminase can modulate the morphological impacts of bacteria on root hair response by affecting plant ethylene content.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
含1-氨基环丙烷-1-羧酸脱氨酶的青花假单胞菌和异花椒对番茄根构和根毛性状的调控
微生物酶ACC脱氨酶通过降低根系1-氨基环丙烷-1-羧酸盐(ACC)含量和植物乙烯产量,是促进植物生长的根细菌(PGPR)的一种广泛的有益特性,可以改善乙烯介导的根系生长抑制。然而,关于细菌ACC脱氨酶是否调节根系结构和根毛性状,目前知之甚少。因此,矮化番茄(Solanum lycopersicum)栽培品种Micro Tom在体外接种假单胞菌brassicacearum Am3、其ACC脱氨酶缺陷突变体T8-1、已知的PGPR菌株Variovorax paradoxus 5C-2,或用促进或抑制乙烯产生或敏感性的试剂(Ag+、Co2+和ACC)进行化学处理。ACC处理降低了根伸长和侧根数量,而乙烯抑制剂(Ag+,Co2+)和V.paradous 5C-2促进了主根伸长,但对侧根长度和数量的影响不同。Ag+对侧根发育有促进作用,而Co2+和V.paradous 5C-2则没有。用P.brassicacearum Am3和T8-1接种在高接种浓度(10 6个细胞cm 3)下抑制主根和侧根的伸长。所有菌株都显著增加了根毛的长度和数量,其中这些影响在甘蓝型油菜Am3中比在突变体T8-1中更明显。Ag+处理抑制根毛的形成和伸长,而Co2+处理则相反。ACC处理对根毛伸长没有影响,但增加了根毛密度。虽然甘蓝型油菜Am3引起的根系生长抑制与ACC脱氨酶无关,但ACC脱氨蛋白酶活性增强了该菌株对根毛伸长和密度的促进作用。因此ACC脱氨酶可以通过影响植物乙烯含量来调节细菌对根毛反应的形态学影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biologia Plantarum
Biologia Plantarum 生物-植物科学
CiteScore
2.80
自引率
0.00%
发文量
28
审稿时长
3.3 months
期刊介绍: BIOLOGIA PLANTARUM is an international journal for experimental botany. It publishes original scientific papers and brief communications, reviews on specialized topics, and book reviews in plant physiology, plant biochemistry and biophysics, physiological anatomy, ecophysiology, genetics, molecular biology, cell biology, evolution, and pathophysiology. All papers should contribute substantially to the current level of plant science and combine originality with a potential general interest. The journal focuses on model and crop plants, as well as on under-investigated species.
期刊最新文献
Evaluating root characteristics under field conditions in perennial ryegrass for potential application in commercial breeding programmes Pod physical traits significantly implicate shattering response of pods in beans (Phaseolus vulgaris L.) Metabolites profiling of five Eucalyptus species by gas chromatography-mass spectrometry and multivariate analysis Differential biomass and nutrient accumulation in perennial ryegrass accessions under excess water treatment in field conditions during winter Optimized tetraploidization strategies in tissue culture for Lolium, Festuca, and Festulolium
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1