Effect of Ozone on Oxygen Transport and Pro-Oxidant- Antioxidant Balance of Red Blood Cell Suspension

Abstract

Introduction: Ozone affects blood oxygen transport and the pro-oxidant-antioxidant balance. However, the role of blood formed elements and gas transmitters in these processes still remains unclear. The aim of the present study was to investigate the effect of ozone on oxygen transport and the pro-oxidant-antioxidant balance in a red cell suspension. Methods: The red cell suspension was incubated with ozone at a concentration of 6 mg/l and substances affecting the synthesis of gas transmitters (nitroglycerin, sodium hydrosulfide). Parameters of blood oxygen transport and pro-oxidant-antioxidant balance were determined. Results: The effect of ozone on blood oxygen transport was found which was manifested in an increase in oxygen partial pressure and the degree of oxygenation. The index of hemoglobin –oxygen affinity p50 actual was raised and a shift of the oxyhemoglobin dissociation curve rightwards was noticed. The addition of the gas transmitter donor, nitric oxide, enhanced the effect of this gas on the parameters of oxygen transport in the erythrocyte suspension Conclusion: We found that ozone induced a change in oxygen-binding properties of the erythrocyte suspension which is a mechanism of action of the above gas on the adaptive processes in the body realized directly at the level of erythrocytes.