Overexpression of rice acyl-CoA-binding protein OsACBP5 protects Brassica napus against seedling infection by fungal phytopathogens

IF 1.8 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Crop & Pasture Science Pub Date : 2023-03-31 DOI:10.1071/CP22347
Saritha Panthapulakkal Narayanan, A. Alahakoon, C. Elliott, D. Russell, P. Taylor, Clive Lo, M. Chye
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Abstract

Abstract Context. Class III acyl-CoA-binding proteins such as those from dicots (Arabidopsis and grapevine) play a role in defence against biotrophic pathogens. The overexpression of the monocot Oryza sativa (rice) OsACBP5 in Arabidopsis and rice has been demonstrated to enhance broad-spectrum disease resistance against selected phytopathogens in OsACBP5-overexpressing (OsACBP5-OE) lines. Aims. We aimed to develop transgenic rapid-cycling Brassica napus (B. napus-RC) and canola cv. Westar OsACBP5-OEs using tissue culture-based Agrobacterium-mediated transformation and to evaluate transgenic plants for resistance against Alternaria blight, blackleg and Sclerotinia rot diseases. Methods. Transgenic B. napus-RC and cv. Westar OsACBP5-OEs were generated through Agrobacterium-mediated transformation using Agrobacterium strain LBA4404 harbouring a plasmid with the rice complementary DNA encoding OsACBP5 driven by the cauliflower mosaic virus 35S promoter. Alternaria blight and blackleg pathogen assays were based on infecting young cotyledons, while detached leaf assay was used to test the tolerance of B. napus plants toward Sclerotinia sclerotiorum. Key results. Average transformation efficiencies of 24.2% and 29.1% were obtained for B. napus-RC and B. napus cv. Westar cotyledons respectively. OsACBP5-OE plants exhibited resistance 5 days after inoculation with Alternaria brassicae, 12 days after inoculation with Leptosphaeria maculans, and 24 h after inoculation with S. sclerotiorum. Conclusions. Transformation of B. napus-RC was shown herein to be an effective trait testing platform for canola. This study also provides an insight into the usefulness of OsACBP5 in enhancing resistance to necrotrophic phytopathogens. Implications. OsACBP5 can be overexpressed in other crops to generate pathogen-resistant varieties.
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水稻酰基辅酶a结合蛋白OsACBP5的过表达可保护甘蓝型油菜免受真菌植物病原体的侵染
抽象的上下文。III类酰基辅酶a结合蛋白,如来自菊苣(拟南芥和葡萄藤)的蛋白,在防御生物营养病原体方面发挥作用。在拟南芥和水稻中,单子叶OsACBP5的过表达(OsACBP5- oe)已被证明可以增强OsACBP5-过表达(OsACBP5- oe)系对特定植物病原体的广谱抗性。目标我们的目的是培育转基因快速循环的甘蓝型油菜(B. napus- rc)和油菜。Westar OsACBP5-OEs利用农杆菌介导的组织培养转化并评价转基因植物对疫病、黑腿病和腐核病的抗性。方法。转基因甘蓝型双歧杆菌rc和cv。Westar OsACBP5- oes是通过农杆菌介导的转化产生的,农杆菌菌株LBA4404含有水稻互补DNA编码OsACBP5的质粒,由花椰菜花叶病毒35S启动子驱动。油菜疫病和黑腿病的病原菌检测方法基于侵染幼嫩子叶,离体叶片检测方法用于检测油菜对菌核菌的耐受性。关键的结果。甘蓝型油菜- rc和甘蓝型油菜cv的平均转化效率分别为24.2%和29.1%。分别为西星子叶。OsACBP5-OE植株在接种油菜互花孢菌5 d、黄斑钩带绦虫12 d、菌核葡萄球菌24 h后均表现出抗性。结论。油菜B. napus-RC的转化是油菜有效的性状检测平台。该研究还揭示了OsACBP5在增强对坏死性植物病原体的抗性方面的作用。的影响。OsACBP5可以在其他作物中过表达,从而产生抗病品种。
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来源期刊
Crop & Pasture Science
Crop & Pasture Science AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
4.20
自引率
15.80%
发文量
111
审稿时长
3 months
期刊介绍: Crop and Pasture Science (formerly known as Australian Journal of Agricultural Research) is an international journal publishing outcomes of strategic research in crop and pasture sciences and the sustainability of farming systems. The primary focus is broad-scale cereals, grain legumes, oilseeds and pastures. Articles are encouraged that advance understanding in plant-based agricultural systems through the use of well-defined and original aims designed to test a hypothesis, innovative and rigorous experimental design, and strong interpretation. The journal embraces experimental approaches from molecular level to whole systems, and the research must present novel findings and progress the science of agriculture. Crop and Pasture Science is read by agricultural scientists and plant biologists, industry, administrators, policy-makers, and others with an interest in the challenges and opportunities facing world agricultural production. Crop and Pasture Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
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