{"title":"LIFT of cell spheroids: Proof of concept","authors":"Artem Antoshin , Ekaterina Minaeva , Polina Koteneva , Maria Peshkova , Polina Bikmulina , Nastasia Kosheleva , Yuri Efremov , Anastasia Shpichka , Vladimir Yusupov , Nikita Minaev , Peter Timashev","doi":"10.1016/j.bprint.2023.e00297","DOIUrl":null,"url":null,"abstract":"<div><p><span>The application of spheroids in </span>tissue engineering<span> has a number of advantages over conventional cell suspensions and 2D cultures. One of the methods for tissue and organ fabrication from spheroids is bioprinting. As one of bioprinting methods, laser-induced forward transfer (LIFT) has received much attention in terms of cell printing, while its potential has not been realized for spheroid patterning yet. In this paper, the authors have shown for the first time the practical applicability of LIFT for spheroid transfer with high survival rates and printing precision. For this, a special optical device, a piShaper, was used to change the laser energy distribution to non-Gaussian profile which allowed for mitigating the negative effects of laser radiation on the spheroids during LIFT. The authors showed that non-Gaussian energy distribution in the laser spot in the form of double ring led to higher post-printing viability of spheroids than in case of conventional Gaussian energy distribution in laser beam. Subsequently, using the double ring laser spot geometry, the spheroids were bioprinted in the form of simple geometric figures: line, triangle, and square. Overall, LIFT bioprinting of spheroids has demonstrated a strong potential as the precise, safe, and reproducible method for biofabrication that can be potentially used for making tissue-engineered bioequivalents or building specific organ-on-a-chip platforms.</span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000404","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
引用次数: 0
Abstract
The application of spheroids in tissue engineering has a number of advantages over conventional cell suspensions and 2D cultures. One of the methods for tissue and organ fabrication from spheroids is bioprinting. As one of bioprinting methods, laser-induced forward transfer (LIFT) has received much attention in terms of cell printing, while its potential has not been realized for spheroid patterning yet. In this paper, the authors have shown for the first time the practical applicability of LIFT for spheroid transfer with high survival rates and printing precision. For this, a special optical device, a piShaper, was used to change the laser energy distribution to non-Gaussian profile which allowed for mitigating the negative effects of laser radiation on the spheroids during LIFT. The authors showed that non-Gaussian energy distribution in the laser spot in the form of double ring led to higher post-printing viability of spheroids than in case of conventional Gaussian energy distribution in laser beam. Subsequently, using the double ring laser spot geometry, the spheroids were bioprinted in the form of simple geometric figures: line, triangle, and square. Overall, LIFT bioprinting of spheroids has demonstrated a strong potential as the precise, safe, and reproducible method for biofabrication that can be potentially used for making tissue-engineered bioequivalents or building specific organ-on-a-chip platforms.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.