{"title":"Application of selective polymeric sorbents for simple coumarins extraction from deodorant samples","authors":"K. Hroboňová, Andrea Špačková","doi":"10.2478/acs-2020-0009","DOIUrl":null,"url":null,"abstract":"Abstract Coumarins (2H-1-benzopyran-2-coumarin derivatives) are derivatives of cinnamic acid naturally occurring in many plants, fungi, and fruits. They are used as ingredients in cosmetics to enhance the aroma and other biological effects. In this work, cosmetic samples (deodorants) were treated by solid phase extraction prior to high performance liquid chromatography determination of coumarins. Traditional sorbent (C18) and selective polymer-based sorbents (laboratory prepared and commercial) were used for solid phase extraction. Recovery values were above 85 % (RSDs below 6 %) except for esculin, where the recovery was lower. Core-shell column of C18 type and gradient of mobile phase methanol—1 % acetic acid were used for high performance liquid chromatography analysis of extracts. Limits of quantitation were 0.5 µg mL−1 for coumarin (ultraviolet detection) and below 12 ng mL−1 for esculin, umbelliferonene, scoparone, 4-methylumbelliferone, herniarin (fluorescence detection). In the tested samples, no coumarins were detected.","PeriodicalId":7088,"journal":{"name":"Acta Chimica Slovaca","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Chimica Slovaca","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/acs-2020-0009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 2
Abstract
Abstract Coumarins (2H-1-benzopyran-2-coumarin derivatives) are derivatives of cinnamic acid naturally occurring in many plants, fungi, and fruits. They are used as ingredients in cosmetics to enhance the aroma and other biological effects. In this work, cosmetic samples (deodorants) were treated by solid phase extraction prior to high performance liquid chromatography determination of coumarins. Traditional sorbent (C18) and selective polymer-based sorbents (laboratory prepared and commercial) were used for solid phase extraction. Recovery values were above 85 % (RSDs below 6 %) except for esculin, where the recovery was lower. Core-shell column of C18 type and gradient of mobile phase methanol—1 % acetic acid were used for high performance liquid chromatography analysis of extracts. Limits of quantitation were 0.5 µg mL−1 for coumarin (ultraviolet detection) and below 12 ng mL−1 for esculin, umbelliferonene, scoparone, 4-methylumbelliferone, herniarin (fluorescence detection). In the tested samples, no coumarins were detected.
摘要香豆素(2H-1-苯并吡喃-2-香豆素衍生物)是肉桂酸的衍生物,天然存在于许多植物、真菌和水果中。它们被用作化妆品中的成分,以增强香气和其他生物效应。在这项工作中,在高效液相色谱法测定香豆素之前,通过固相萃取处理化妆品样品(除臭剂)。传统吸附剂(C18)和选择性聚合物基吸附剂(实验室制备和商业化)用于固相萃取。回收率高于85%(RSD低于6%),但埃斯库林的回收率较低。采用C18型核壳柱,流动相甲醇-1%乙酸梯度洗脱,对提取物进行高效液相色谱分析。香豆素(紫外检测)的定量限为0.5µg mL−1,而esculin、伞形花烯、东莨菪碱、4-甲基伞形花烯和疝环素(荧光检测)的含量限低于12 ng mL−1。在测试样品中,未检测到香豆素。