Garcinia kola treatment exhibits immunomodulatory properties while not affecting type 1 diabetes development in an experimental mouse model

M. Cetkovic-Cvrlje, S. Rogan, Emily Barbaro
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引用次数: 1

Abstract

Objective T cells orchestrate an inflammatory response that destroys pancreatic insulin-producing β cells during the development of autoimmune type 1 diabetes (T1D). Garcinia kola Heckel (GK) is a plant widely exploited in West African traditional medicine. Some of the therapeutic effects of GK nut’s extract (GKE) have been suggested to be due to its anti-inflammatory potential. Since GKE has never been investigated in a T1D experimental model, nor in the T cells’ context, we aimed to determine whether GKE exhibits antidiabetic properties and affects T cells by its anticipated anti-inflammatory action. Methods The effect of aqueous GKE (aGKE) ingestion, 100 mg/kg daily by drinking water over the period of 6 weeks, has been tested in a low-dose streptozotocin-induced (LDSTZ) mouse model of autoimmune T1D. T cells were studied in vitro and in vivo in mice treated by aGKE. Results The results showed that aGKE treatment, which started a week before induction of disease, neither delayed the development of T1D, nor reduced glycemia severity. Interestingly, aGKE treatment did affect T cells and their function, significantly decreasing the frequency of helper (TH) and cytotoxic (TC) T cells, while elevating the levels of pro-inflammatory cytokines, TNF-α, IL-6, and IFN-γ, and suppressing IL-2. Conclusion In conclusion, our results did not confirm the antidiabetic property of GKE, while suggesting its therapeutic exploration in TH2-dependent pathologies that benefit from an aggravated TH1 response, such as allergies.
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在实验小鼠模型中,藤黄治疗显示出免疫调节特性,同时不影响1型糖尿病的发展
目的在自身免疫性1型糖尿病(T1D)的发展过程中,T细胞协调破坏胰腺胰岛素产生β细胞的炎症反应。藤黄(GK)是西非传统医药中广泛使用的一种植物。GK坚果提取物(GKE)的一些治疗作用被认为是由于其抗炎潜力。由于GKE从未在T1D实验模型中进行过研究,也从未在T细胞中进行过调查,我们旨在确定GKE是否具有抗糖尿病特性,并通过其预期的抗炎作用影响T细胞。方法在低剂量链脲佐菌素诱导(LDSTZ)的自身免疫性T1D小鼠模型中,通过饮水每天摄入100mg/kg的GKE(aGKE),持续6周。在用aGKE处理的小鼠中对T细胞进行了体外和体内研究。结果aGKE治疗在疾病诱导前一周开始,既没有延缓T1D的发展,也没有降低血糖的严重程度。有趣的是,aGKE治疗确实影响了T细胞及其功能,显著降低了辅助性(TH)和细胞毒性(TC)T细胞的频率,同时提高了促炎细胞因子、TNF-α、IL-6和IFN-γ的水平,并抑制了IL-2。结论总之,我们的研究结果并没有证实GKE的抗糖尿病特性,同时表明它在TH2依赖性疾病中的治疗探索,这些疾病受益于TH1反应加重,如过敏。
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来源期刊
CiteScore
4.00
自引率
0.00%
发文量
88
审稿时长
15 weeks
期刊介绍: International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.
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