Chromatographic Profiles Analysis of Fruits of Crataegus L. Genus by High-Performance Thin-Layer Chromatography

European Pharmaceutical Journal Pub Date : 2019-11-01 DOI:10.2478/afpuc-2019-0020

K. Khokhlova, O. Zdoryk, N. Sydora, V.I. Shatrovska

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引用次数: 5

Abstract

Abstract It was known that hawthorn - Crataegus L. is a polymorphic genus. Two hawthorn species and their hybrids are included in the European Pharmacopoeia, twelve – in Ukrainian pharmacopoeia. Determination of chromatographic profiles of hawthorn fruits species native to Ukraine and other countries that are non-pharmacopoeial, but have sufficient plant raw material base, is essential for quality control of drugs. Aim. To analyze and compare the chromatographic profiles of fruits of 23 Crataegus L. species on phenolic compounds, evaluated by means of high-performance thin-layer chromatography procedure (HPTLC), and determine the specific features of chromatographic fingerprints. Materials and Methods. A total of 39 samples of fruits of 23 hawthorn species that are native to Europe, Asia and North America, such as Crataegus monogyna, C. laevigata/C. oxyacantha, C. leiomonogyna, C. curvisepala, C. pseudokyrtostyla, C. fallacina, C. subrotunda, C. ambigua, C. pentagyna, C. sanguinea, C. chlorosarca, C. almaatensis, C.pseudoheterophylla subsp. turkestanica, C. pinnatifida, C. pentagyna subsp. pseudomelanocarpa, C. punctata, C. pringlei, C. festiva, C. douglasii, C. holmesiana, C. submollis, C. flabellata, C. canadensis were investigated. The analysis has been done following the TLC method from European Pharmacopeia modified into HPTLC, using automated HPTLC herbal system (CAMAG, Switzerland). The results have shown that chromatographic profiles of phenolic constituents of nine Crataegus L. species of Europe, both pharmacopoeial and non-pharmacopoeial, were quite similar, despite the significant morphological distinctions. The chromatographical profiles of three species of Asia were similar to the pharmacopoeial species; three other species looked different and had specific marker zones. In addition, eight Crataegus L. species of North America had specific markers helping for discriminative analysis from pharmacopoeial species. Conclusion. The findings could help to identify the possible adulterations and prevent the falsification of finished products. The results will be taken into consideration during revision of the Ukrainian national pharmacopoeial monograph for hawthorn fruits.

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高效薄层色谱法分析山楂属果实的色谱特征

摘要山楂属(hawthorn - Crataegus L.)是一个多态属。欧洲药典收录了两种山楂及其杂交种，乌克兰药典收录了12种。乌克兰等国原产的山楂属非药典品种，但具有充足的植物原料基础，其色谱图谱的测定对药品质量控制至关重要。的目标。采用高效薄层色谱法(HPTLC)对23种山楂果实中酚类化合物的色谱图谱进行分析比较，并确定其色谱指纹图谱特征。材料与方法。本文收集了原产于欧洲、亚洲和北美的山楂(Crataegus monogyna, C. laevigata/C.)等23种山楂的39份果实样本。氧棘、扁桃花、曲叶花、假柱头花、fallacina、圆底花、双歧花、五角花、血根花、绿根花、almaatensis、假异叶花。土耳其，山裙花，五角草亚属。调查了假黑桫椤、斑点桫椤、春花桫椤、假日桫椤、道格拉斯桫椤、美洲桫椤、亚mollis、flabellata和加拿大桫椤。采用全自动HPTLC草药分析系统(CAMAG，瑞士)，将欧洲药典的TLC方法修改为HPTLC。结果表明，欧洲9种山楂属植物(药典种和非药典种)的酚类成分色谱图谱基本一致，但形态上存在显著差异。3个亚洲种的色谱图谱与药典种相似;另外三个物种看起来不同，有特定的标记区。另外，北美8种山楂属植物具有特异性标记物，可用于药典种间的鉴别分析。结论。研究结果可以帮助识别可能的掺假，防止成品伪造。在修订乌克兰山楂果国家药典各论时将考虑到这些结果。

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来源期刊

European Pharmaceutical Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

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0.60

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0.00%

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期刊介绍： European Pharmaceutical Journal publishes only original articles not previously published and articles that are not being considered or have not been submitted for publication elsewhere. If parts of the results have been published as conference abstract or elsewhere, it should be stated in references. The ethical standards of the Helsinki-Tokio Declaration should be kept. This should be mentioned in the Methods of manuscript. Reviews are published only on request. Authors, whose submitted research work was performed with the support of a company, should indicate this in Conflict of Interest.