A Case-Control Study of the Luteinizing Hormone Level in Luteinizing Hormone Receptor Gene (rs2293275) Polymorphism in Polycystic Ovarian Syndrome Females.

IF 1.3 4区医学 Q4 GENETICS & HEREDITY Public Health Genomics Pub Date : 2022-03-29 DOI:10.1159/000521971

Manar Fayiz Atoum, Mai Mahamad Alajlouni, Foad Alzoughool

{"title":"A Case-Control Study of the Luteinizing Hormone Level in Luteinizing Hormone Receptor Gene (rs2293275) Polymorphism in Polycystic Ovarian Syndrome Females.","authors":"Manar Fayiz Atoum, Mai Mahamad Alajlouni, Foad Alzoughool","doi":"10.1159/000521971","DOIUrl":null,"url":null,"abstract":"Background: Polycystic ovary syndrome (PCOS) is a common heterogeneous disorder characterized by chronic anovulation, infertility, polycystic ovaries, and hyperandrogenic signs.Objective: The aim of this study was to determine the association of luteinizing hormone/chorionic gonadotropin hormone receptor LHCGR polymorphism (rs2293275) with oligomenorrhea, amenorrhea, hirsutism, acne, infertility, LH, LH/FSH ratio, and body mass index (BMI) among PCOS females.Methods: This genetic case-control study recruited 55 PCOS and 55 control females, diagnosed based on the Rotterdam criteria. LH and FSH were measured by the Roche cobas c 502 automated analyzer. Genotypic analysis was carried out using the polymerase chain reaction-restriction fragment length polymorphism and restriction endonuclease digestion.Results: BMI was higher for PCOS patients (28.5 ± 6.59) compared to controls (25.1 ± 5.77), and ovulatory dysfunction was seen among 90% of PCOS females. Oligomenorrhea was common in PCOS (73%), and hirsutism and acne were detected in PCOS (80% and 40%; respectively). LH ≥10 were recoded among 51%, while LH/FSH ≥1.5 was recorded among 33% PCOS females. There is a statistical difference between rs2293275 polymorphism in the AG genotype between PCOS patients and controls. PCOS patients have a significantly higher mean LH level compared to controls (8.36 ± 4.86 and 5.67 ± 2.51, respectively) and showed higher LH/FSH value (1.46 ± 0.81) compared to (0.87 ± 0.30) controls. GG and AG genotypes of LHCGR showed statistically significant higher LH (8.22 ± 4.11; 9.02 ± 3.87) and LH/FSH values (1.57 ± 0.56; 1.64 ± 0.89) compared to controls.Conclusion: LHCGR (rs2293275) GA and GG genetic variants could modulate the hormonal levels of PCOS LH levels and the LH/FSH ratio and associated with hirsutism, oligomenorrhea, BMI, and LH/FSH ratio as risk factors.","PeriodicalId":49650,"journal":{"name":"Public Health Genomics","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Public Health Genomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000521971","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Polycystic ovary syndrome (PCOS) is a common heterogeneous disorder characterized by chronic anovulation, infertility, polycystic ovaries, and hyperandrogenic signs.

Objective: The aim of this study was to determine the association of luteinizing hormone/chorionic gonadotropin hormone receptor LHCGR polymorphism (rs2293275) with oligomenorrhea, amenorrhea, hirsutism, acne, infertility, LH, LH/FSH ratio, and body mass index (BMI) among PCOS females.

Methods: This genetic case-control study recruited 55 PCOS and 55 control females, diagnosed based on the Rotterdam criteria. LH and FSH were measured by the Roche cobas c 502 automated analyzer. Genotypic analysis was carried out using the polymerase chain reaction-restriction fragment length polymorphism and restriction endonuclease digestion.

Results: BMI was higher for PCOS patients (28.5 ± 6.59) compared to controls (25.1 ± 5.77), and ovulatory dysfunction was seen among 90% of PCOS females. Oligomenorrhea was common in PCOS (73%), and hirsutism and acne were detected in PCOS (80% and 40%; respectively). LH ≥10 were recoded among 51%, while LH/FSH ≥1.5 was recorded among 33% PCOS females. There is a statistical difference between rs2293275 polymorphism in the AG genotype between PCOS patients and controls. PCOS patients have a significantly higher mean LH level compared to controls (8.36 ± 4.86 and 5.67 ± 2.51, respectively) and showed higher LH/FSH value (1.46 ± 0.81) compared to (0.87 ± 0.30) controls. GG and AG genotypes of LHCGR showed statistically significant higher LH (8.22 ± 4.11; 9.02 ± 3.87) and LH/FSH values (1.57 ± 0.56; 1.64 ± 0.89) compared to controls.

Conclusion: LHCGR (rs2293275) GA and GG genetic variants could modulate the hormonal levels of PCOS LH levels and the LH/FSH ratio and associated with hirsutism, oligomenorrhea, BMI, and LH/FSH ratio as risk factors.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

多囊卵巢综合征女性促黄体激素水平与促黄体激素受体基因(rs2293275)多态性的病例对照研究

背景：多囊卵巢综合征（PCOS）是一种常见的异质性疾病，以慢性无排卵、不孕、多囊卵巢和高雄激素综合征为特征。目的：本研究的目的是确定多囊卵巢综合征女性黄体生成素/绒毛膜促性腺激素受体LHCGR多态性（rs2293275）与月经过少、闭经、多毛、痤疮、不孕、LH、LH/FSH比值和体重指数（BMI）的关系。方法：这项基因病例对照研究招募了55名PCOS和55名对照女性，根据鹿特丹标准进行诊断。LH和FSH用Roche cobas c 502全自动分析仪测定。利用聚合酶链式反应限制性片段长度多态性和限制性内切酶切进行基因型分析。结果：PCOS患者的BMI（28.5±6.59）高于对照组（25.1±5.77），90%的PCOS女性出现排卵功能障碍。月经过少在多囊卵巢综合征中常见（73%），多毛和痤疮在多囊卵巢综合症中常见（分别为80%和40%）。在51%的PCOS女性中记录到LH≥10，而在33%的PCOS妇女中记录到LH/FSH≥1.5。多囊卵巢综合征患者和对照组AG基因型rs2293275多态性之间存在统计学差异。PCOS患者的平均LH水平显著高于对照组（分别为8.36±4.86和5.67±2.51），LH/FSH值（1.46±0.81）高于（0.87±0.30）对照组。LHCGR的GG和AG基因型显示出与对照组相比具有统计学意义的更高LH（8.22±4.11；9.02±3.87）和LH/FSH值（1.57±0.56；1.64±0.89）。结论：LHCGR（rs2293275）GA和GG基因变异可调节PCOS激素水平LH水平和LH/FSH比值，并与多毛症、月经过少、BMI和LH/FFSH比值相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Public Health Genomics 医学-公共卫生、环境卫生与职业卫生

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： ''Public Health Genomics'' is the leading international journal focusing on the timely translation of genome-based knowledge and technologies into public health, health policies, and healthcare as a whole. This peer-reviewed journal is a bimonthly forum featuring original papers, reviews, short communications, and policy statements. It is supplemented by topic-specific issues providing a comprehensive, holistic and ''all-inclusive'' picture of the chosen subject. Multidisciplinary in scope, it combines theoretical and empirical work from a range of disciplines, notably public health, molecular and medical sciences, the humanities and social sciences. In so doing, it also takes into account rapid scientific advances from fields such as systems biology, microbiomics, epigenomics or information and communication technologies as well as the hight potential of ''big data'' for public health.