Comparative characterization and expression analysis revealed cinnamyl alcohol dehydrogenase genes differentially responding to Colletotrichum fructicola in woodland and cultivated strawberries

IF 1.5 4区农林科学 Q3 PLANT SCIENCES Journal of Berry Research Pub Date : 2023-06-09 DOI:10.3233/jbr-230012

Zi-Yi Li, K. Duan, Jiayue Zhu, Ling Bin, Xinyi Wang, Si-Yu Chen, Xue Li, Chunnu Geng, Q. Gao

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Abstract

BACKGROUND: Strawberry (Fragaria×ananassa) production is hampered by anthracnose caused by Colletotrichum spp. Cinnamyl alcohol dehydrogenase (CAD) catalyzes lignin biosynthesis to fortify plant cell wall upon various stresses, but their involvement in strawberry resistance is elusive. OBJECTIVE: Current work aims to uncover the involvement of CAD genes in both woodland and cultivated strawberry responses to Colletotrichum spp. METHODS: Genome-wide identification of CAD genes was updated in cultivated strawberry. Comparative molecular characterization integrated with pathogenesis phenotyping after C. fructicola inoculation, were followed by RT-qPCR analysis and RNAseq data retrieval. RESULTS: Cultivated strawberry comprises 32 CAD genes of three classes. Thirteen syntenic relationships were observed between cultivated and woodland strawberry, with high homology in protein coding regions while promoters being greatly diversified. F. vesca ‘Hawaii4’ was least susceptible, followed by octoploid cultivar ‘ShenQi’, and ‘Benihoppe’ the most susceptible to C. fructicola. The class I CAD4 and CAD5, preferentially expressed in leaves and crowns, respectively, were up-regulated in susceptible varieties at 72 h post inoculation (hpi). Of class II, CAD8 was up-regulated in three genotypes at distinct timepoints, while CAD12 was quickly down-regulated in ‘ShenQi’ upon inoculation. CAD1 and CAD2 of class III were down-regulated in ‘ShenQi’ and ‘Hawaii4’ at distinct stages. The strong involvement of CAD genes in strawberry response to Colletotrichum spp. sets a basis for future functional study. CONCLUSION: F. vesca is less susceptible to Colletotrichum spp. than cultivated strawberries but the expression of CAD family in F. vesca and F.×ananassa were differentially provoked in timing and magnitude hinting no correlation with strawberry resistance to this pathogen.

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比较表征和表达分析揭示了肉桂醇脱氢酶基因对林地和栽培草莓炭疽病的不同反应

背景：炭疽菌引起的炭疽病阻碍了草莓（Fragaria×ananassa）的生产。肉桂醇脱氢酶（CAD）在各种胁迫下催化木质素生物合成以增强植物细胞壁，但它们与草莓抗性的关系尚不清楚。目的：目前的工作旨在揭示CAD基因在林地和栽培草莓对炭疽菌的反应中的作用。方法：更新栽培草莓CAD基因的全基因组鉴定。将比较分子特征与接种后的发病机制表型相结合，然后进行RT-qPCR分析和RNAseq数据检索。结果：栽培草莓含有3类32个CAD基因。在栽培草莓和林地草莓之间观察到13个同基因关系，在蛋白质编码区具有高度同源性，而启动子则非常多样化。黄颡鱼‘Hawaii4’最不易感，其次是八倍体品种‘ShenQi’，而‘Benihope’最易感。I类CAD4和CAD5分别优先在叶片和树冠中表达，在72岁时在易感品种中上调接种后h（hpi）。在II类中，CAD8在三种基因型中在不同的时间点上调，而CAD12在“神芪”中在接种后迅速下调。Ⅲ类CAD1和CAD2在‘申启’和‘夏威夷’不同阶段均下调。CAD基因在草莓对炭疽菌的反应中的强烈参与为未来的功能研究奠定了基础。结论：与栽培草莓相比，vesca对Colletotrichum spp.的敏感性较低，但CAD家族在vesca和×ananassa中的表达在时间和数量上存在差异，这与草莓对该病原体的抗性无关。

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来源期刊

Journal of Berry Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

3.50

自引率

11.80%

发文量

期刊介绍： The main objective of the Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems. The objective will be achieved by focusing on four main areas of research and development: From genetics to variety evaluation Nursery production systems and plant quality control Plant physiology, biochemistry and molecular biology, as well as cultural management Health for the consumer: components and factors affecting berries'' nutritional value Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.