Protective Effect of Resveratrol on Vincristine-induced Oxidative Stress in Mouse Ovary and UterineTissue

Abstract

Background & objectives: Vincristine (VIN) is a broad-spectrum anticancer drug has been used to treat various cancers. Resveratrol (Res) is a natural polyphenol found in many plant sources. Many studies have reported anti-inflammatory and antioxidative effects of resveratrol. We have explored the protective effect of resveratrol on vincristine-induced oxidative stress in mouse ovarian tissue. Methods: In this study, 32 female mice weighing 25-30 grams were randomly divided into four groups (each group n=8): 1control group, 2Vingroup, 3Vin-Res group and 4Res group. The mice received a single IP injection of vincristine (3 mg/kg) weekly for 4 weeks. Res treatment was done 28 days by gastric gavage (daily 30 mg/kg).At the end of the study, Malondialdehyde (MDA) and total antioxidant capacity (TAC) and the antioxidant activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes were measured in ovarian tissue and uterus of the studied animals. Also, ovarian follicles were counted. Results: The results indicated that the MDA level was elevated and TAC, GPx as well as SOD activities were decreased in Vingroup significantly. Resveratrol reduced MDA level and increased GPx and SOD activities in Vin-Res group significantly. Also histological findings showed that Res increased primordial and primary follicles and reduced atretic follicles in Vin-Res group significantly. Conclusions: These results indicate the protective effect of resveratrol on ovarian and uterine tissue against oxidative damage of vincristine in mice.