Purification, structural elucidation and immunostimulatory effect of a new protein-polysaccharide conjugate produced by Nervilia fordii

IF 1.2 4区化学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Carbohydrate Chemistry Pub Date : 2021-01-01 DOI:10.1080/07328303.2021.1975733

Jizhao Xie , Li Qiu , Luhui Zou , Yunfeng Xie , Di Luo , Huanji Xu , Xinduo Wu , Lisheng Wang

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Abstract

A novel protein-polysaccharide conjugate NFP-A4 was isolated and purified from water extracts of Nervilia fordii. The polysaccharide chain of NFP-A4 was found to contain D-galacturonic acid, D-galactose, D-glucose, L-arabinose, D-glucuronic acid, L-rhamnose, and D-mannose. The protein content of NFP-A4 was determined to be 12.83%, and it was bound to the polysaccharide chain via O-linkages at the serine and threonine residues. NFP-A4 was found to significantly activate macrophages and also induce immunomodulatory responses. These data suggest that NFP-A4 is a major bioactive component of N. fordii and may be responsible for many of its therapeutic properties.

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一种新的油桐蛋白-多糖结合物的纯化、结构分析及免疫刺激作用

从油桐水提物中分离纯化了一种新的蛋白-多糖偶联物NFP-A4。发现NFP-A4的多糖链含有d -半乳糖、d -半乳糖、d -葡萄糖、l -阿拉伯糖、d -葡萄糖醛酸、l -鼠李糖和d -甘露糖。测定NFP-A4的蛋白质含量为12.83%，在丝氨酸和苏氨酸残基上通过o键结合到多糖链上。发现NFP-A4能显著激活巨噬细胞并诱导免疫调节反应。这些数据表明，NFP-A4是福氏弓形虫的主要生物活性成分，可能是其许多治疗特性的原因。下载:下载高清图片(370KB)下载:下载全尺寸图片

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来源期刊

Journal of Carbohydrate Chemistry 化学-生化与分子生物学

CiteScore

2.10

自引率

0.00%

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审稿时长

1 months

期刊介绍： The Journal of Carbohydrate Chemistry serves as an international forum for research advances involving the chemistry and biology of carbohydrates. The following aspects are considered to fall within the scope of this journal: -novel synthetic methods involving carbohydrates, oligosaccharides, and glycoconjugates- the use of chemical methods to address aspects of glycobiology- spectroscopic and crystallographic structure studies of carbohydrates- computational and molecular modeling studies- physicochemical studies involving carbohydrates and the chemistry and biochemistry of carbohydrate polymers.