Massive LINE-1 retrotransposon enrichment in tamarins of the Cebidae family (Platyrrhini, Primates) and its significance for genome evolution

Abstract

To study heterochromatin distribution differences among tamarins, we applied LINE-1 probes using fluorescence in situ hybridization onto chromosomes of Saguinus mystax, Leontocebus fuscicollis, and Leontopithecus rosalia with the aim to investigate possible evolutionary implications. LINE-1 repeats were shown to be involved in genome architecture and in the occurrence of chromosomal rearrangements in many vertebrates. We found bright LINE-1 probe signals at centromeric or pericentromeric areas, GC rich, on almost all chromosomes in three tamarin species. We also found non-centromeric signals along chromosome arms. In a phylogenetic perspective, we analyzed the pattern of LINE-1 distribution considering human chromosomal homologies and C banding patterns. Our data indicate that LINE-1 centromeric expansions and accumulation presumably arose in a common tamarin ancestor and that the presence of LINE-1 at the junction of human chromosome associations is presumably linked to interchromosomal rearrangements. For example, we found bright centromeric signals as well as non-centromeric signals on chromosomes 1 and 2, in all species analyzed, in correspondence to human chromosome associations 13/9/22 and 20/17/13, which are synapomorphic for all tamarins. Furthermore, we found other faint signals that could be apomorphisms linked both to intrachromosomal rearrangements as well as to retro-transposition events. Our results confirm that the three species have similar karyotypes but small differences in LINE-1 and heterochromatin amplification and distribution; in particular on chromosome pairs 19–22, where we show the occurrence of small inversions, in agreement with previous classic cytogenetic hypotheses.